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Literature summary for 1.8.3.1 extracted from
- Structure-based alteration of substrate specificity and catalytic activity of sulfite oxidase from sulfite oxidation to nitrate reduction (2012), Biochemistry, 51, 1134-1147.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
- |
Gallus gallus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R472M | introduction of predicted catalytic site residues of assimilatory nitrate reductase, kinetic analysis | Homo sapiens |
| R472Q | introduction of predicted catalytic site residues of assimilatory nitrate reductase, kinetic analysis | Homo sapiens |
| V474M | active site mutant, kinetic analysis | Homo sapiens |
| Y322F/R450M | introduction of predicted catalytic site residues of assimilatory nitrate reductase, markedly decreased ability to bind sulfite at pH 8.5 | Gallus gallus |
| Y343F/R472Q | active site mutant, kinetic analysis | Homo sapiens |
| Y343N | active site mutant, kinetic analysis | Homo sapiens |
| Y343N/R472M | active site mutant, kinetic analysis | Homo sapiens |
| Y343N/R472M/V474M | active site mutant, kinetic analysis | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00046 | - |
sulfite | mutant V474M, 25°C, pH 6.0 | Homo sapiens |  |
| 0.0013 | - |
sulfite | wild-type, 25°C, pH 6.0 | Homo sapiens | |
| 0.00133 | - |
sulfite | wild-type, 25°C, pH 7.0 | Gallus gallus | |
| 0.00134 | - |
sulfite | mutant V474M, 25°C, pH 7.0 | Homo sapiens | |
| 0.0019 | - |
sulfite | mutant R472M, 25°C, pH 6.0 | Homo sapiens | |
| 0.0023 | - |
sulfite | mutant R472Q, 25°C, pH 6.0 | Homo sapiens | |
| 0.0025 | - |
sulfite | mutant R472M, 25°C, pH 7.0 | Homo sapiens | |
| 0.0027 | - |
sulfite | wild-type, 25°C, pH 7.0 | Homo sapiens | |
| 0.0032 | - |
sulfite | mutant V474M, 25°C, pH 8.0 | Homo sapiens | |
| 0.0035 | - |
sulfite | mutant R472Q, 25°C, pH 7.0 | Homo sapiens | |
| 0.0043 | - |
sulfite | wild-type, 25°C, pH 8.0 | Homo sapiens | |
| 0.0047 | - |
sulfite | mutant R472M, 25°C, pH 8.0 | Homo sapiens | |
| 0.008 | - |
sulfite | wild-type, 25°C, pH 8.5 | Gallus gallus | |
| 0.0083 | - |
sulfite | wild-type, 25°C, pH 8.5 | Homo sapiens | |
| 0.013 | - |
sulfite | mutant R472Q, 25°C, pH 8.0 | Homo sapiens | |
| 0.0144 | - |
sulfite | mutant V474M, 25°C, pH 9.0 | Homo sapiens | |
| 0.016 | - |
sulfite | mutant R472Q, 25°C, pH 8.5 | Homo sapiens | |
| 0.021 | - |
sulfite | mutant R472M, 25°C, pH 9.0 | Homo sapiens | |
| 0.022 | - |
sulfite | wild-type, 25°C, pH 9.0 | Homo sapiens | |
| 0.0319 | - |
sulfite | mutant V474M, 25°C, pH 9.5 | Homo sapiens | |
| 0.0354 | - |
sulfite | mutant V474M, 25°C, pH 8.5 | Homo sapiens | |
| 0.04 | - |
sulfite | mutant V474M, 25°C, pH 10.0 | Homo sapiens | |
| 0.045 | - |
sulfite | mutant R472Q, 25°C, pH 9.0 | Homo sapiens | |
| 0.0529 | - |
sulfite | wild-type, 25°C, pH 10.0 | Homo sapiens | |
| 0.0537 | - |
sulfite | mutant R472M, 25°C, pH 9.5 | Homo sapiens | |
| 0.0614 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 6.0 | Homo sapiens | |
| 0.067 | - |
sulfite | wild-type, 25°C, pH 9.5 | Homo sapiens | |
| 0.0877 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 7.0 | Homo sapiens | |
| 0.094 | - |
sulfite | mutant R472M, 25°C, pH 8.5 | Homo sapiens | |
| 0.0947 | - |
sulfite | mutant Y343N, 25°C, pH 7.0 | Homo sapiens | |
| 0.0953 | - |
sulfite | mutant Y343N, 25°C, pH 6.0 | Homo sapiens | |
| 0.0967 | - |
sulfite | mutant R472Q, 25°C, pH 9.5 | Homo sapiens | |
| 0.0969 | - |
sulfite | mutant R472M, 25°C, pH 10.0 | Homo sapiens | |
| 0.181 | - |
sulfite | mutant R472Q, 25°C, pH 10.0 | Homo sapiens | |
| 0.2827 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.0 | Homo sapiens | |
| 0.297 | - |
sulfite | mutant Y343N, 25°C, pH 8.0 | Homo sapiens | |
| 0.712 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.5 | Homo sapiens | |
| 0.85 | - |
sulfite | mutant Y343N, 25°C, pH 8.5 | Homo sapiens | |
| 1.1 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 6.0 | Homo sapiens | |
| 1.42 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 7.0 | Homo sapiens | |
| 2.14 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.0 | Homo sapiens | |
| 2.46 | - |
sulfite | mutant Y343N, 25°C, pH 9.0 | Homo sapiens | |
| 3.34 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.0 | Homo sapiens | |
| 4.64 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 7.0 | Homo sapiens | |
| 8.6 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 7.0 | Gallus gallus | |
| 9.37 | - |
sulfite | mutant Y343N, 25°C, pH 9.5 | Homo sapiens | |
| 11.73 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 8.5 | Gallus gallus | |
| 12 | - |
sulfite | mutant Y343N, 25°C, pH 10.0 | Homo sapiens | |
| 14 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.5 | Homo sapiens | |
| 16.8 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 6.0 | Homo sapiens | |
| 19.28 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 8.0 | Homo sapiens | |
| 39.9 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.5 | Homo sapiens | |
| 42.99 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 8.5 | Homo sapiens | |
| 55.5 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.0 | Homo sapiens | |
| 59.6 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 10.0 | Homo sapiens | |
| 85.64 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 9.0 | Homo sapiens | |
| 111 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.5 | Homo sapiens | |
| 208 | - |
sulfite | mutant Y343N/R472m, 25°C, pH 9.5 | Homo sapiens | |
| 418 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 10.0 | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | P07850 | - |
- |
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + cytochrome c | - |
Homo sapiens | sulfate + reduced cytochrome c | - |
? | |
| sulfite + cytochrome c | - |
Gallus gallus | sulfate + reduced cytochrome c | - |
? | |
| sulfite + ferricyanide + H2O | - |
Homo sapiens | sulfate + ferrocyanide | - |
? | |
| sulfite + ferricyanide + H2O | - |
Gallus gallus | sulfate + ferrocyanide | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
sulfite | mutant R472M, 25°C, pH 10.0 | Homo sapiens | |
| 0.52 | - |
sulfite | mutant R472Q, 25°C, pH 10.0 | Homo sapiens | |
| 0.97 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 9.5 | Homo sapiens | |
| 1.13 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 7.0 | Homo sapiens | |
| 1.13 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 9.0 | Homo sapiens | |
| 1.33 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 8.0 | Homo sapiens | |
| 1.35 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 6.0 | Homo sapiens | |
| 1.42 | - |
sulfite | mutant Y343N/R472Q, 25°C, pH 8.5 | Homo sapiens | |
| 1.44 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 6.0 | Homo sapiens | |
| 1.7 | - |
sulfite | mutant R472Q, 25°C, pH 9.5 | Homo sapiens | |
| 1.73 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 7.0 | Homo sapiens | |
| 1.8 | - |
sulfite | mutant R472M, 25°C, pH 9.5 | Homo sapiens | |
| 1.9 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 6.0 | Homo sapiens | |
| 2.05 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.0 | Homo sapiens | |
| 2.25 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.5 | Homo sapiens | |
| 2.3 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.0 | Homo sapiens | |
| 2.52 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 8.5 | Gallus gallus | |
| 2.8 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 10.0 | Homo sapiens | |
| 2.96 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 10.0 | Homo sapiens | |
| 3.17 | - |
sulfite | mutant Y343N, 25°C, pH 6.0 | Homo sapiens | |
| 3.4 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 7.0 | Homo sapiens | |
| 3.45 | - |
sulfite | mutant R472M, 25°C, pH 9.0 | Homo sapiens | |
| 3.5 | - |
sulfite | mutant R472M, 25°C, pH 8.0 | Homo sapiens | |
| 3.58 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.0 | Homo sapiens | |
| 3.6 | - |
sulfite | mutant R472M, 25°C, pH 7.0 | Homo sapiens | |
| 3.8 | - |
sulfite | mutant R472M, 25°C, pH 8.5 | Homo sapiens | |
| 3.9 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.5 | Homo sapiens | |
| 4.24 | - |
sulfite | mutant R472Q, 25°C, pH 9.0 | Homo sapiens | |
| 4.38 | - |
sulfite | mutant Y343N, 25°C, pH 10.0 | Homo sapiens | |
| 4.6 | - |
sulfite | mutant R472Q, 25°C, pH 8.5 | Homo sapiens | |
| 4.83 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 7.0 | Gallus gallus | |
| 4.9 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.5 | Homo sapiens | |
| 5 | - |
sulfite | mutant R472M, 25°C, pH 6.0 | Homo sapiens | |
| 5.23 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.5 | Homo sapiens | |
| 5.6 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.0 | Homo sapiens | |
| 5.8 | - |
sulfite | mutant R472Q, 25°C, pH 7.0 | Homo sapiens | |
| 5.96 | - |
sulfite | mutant V474M, 25°C, pH 6.0 | Homo sapiens | |
| 8.1 | - |
sulfite | mutant Y343N, 25°C, pH 9.5 | Homo sapiens | |
| 8.2 | - |
sulfite | mutant R472Q, 25°C, pH 6.0 | Homo sapiens | |
| 9.3 | - |
sulfite | mutant R472Q, 25°C, pH 8.0 | Homo sapiens | |
| 11.4 | - |
sulfite | mutant V474M, 25°C, pH 7.0 | Homo sapiens | |
| 12.4 | - |
sulfite | mutant V474M, 25°C, pH 10.0 | Homo sapiens | |
| 12.8 | - |
sulfite | mutant Y343N, 25°C, pH 7.0 | Homo sapiens | |
| 13 | - |
sulfite | wild-type, 25°C, pH 10.0 | Homo sapiens | |
| 13.2 | - |
sulfite | wild-type, 25°C, pH 6.0 | Homo sapiens | |
| 13.75 | - |
sulfite | mutant Y343N, 25°C, pH 8.0 | Homo sapiens | |
| 15.5 | - |
sulfite | mutant Y343N, 25°C, pH 9.0 | Homo sapiens | |
| 15.8 | - |
sulfite | mutant V474M, 25°C, pH 8.5 | Homo sapiens | |
| 16.9 | - |
sulfite | mutant Y343N, 25°C, pH 8.5 | Homo sapiens | |
| 17.1 | - |
sulfite | mutant V474M, 25°C, pH 9.5 | Homo sapiens | |
| 17.8 | - |
sulfite | mutant V474M, 25°C, pH 8.0 | Homo sapiens | |
| 19.6 | - |
sulfite | mutant V474M, 25°C, pH 9.0 | Homo sapiens | |
| 24.2 | - |
sulfite | wild-type, 25°C, pH 7.0 | Homo sapiens | |
| 25.7 | - |
sulfite | wild-type, 25°C, pH 9.0 | Homo sapiens | |
| 25.9 | - |
sulfite | wild-type, 25°C, pH 8.0 | Homo sapiens | |
| 26.3 | - |
sulfite | wild-type, 25°C, pH 9.5 | Homo sapiens | |
| 26.9 | - |
sulfite | wild-type, 25°C, pH 8.5 | Homo sapiens | |
| 36.1 | - |
sulfite | wild-type, 25°C, pH 7.0 | Gallus gallus | |
| 73 | - |
sulfite | wild-type, 25°C, pH 8.5 | Gallus gallus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00047 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 9.5 | Homo sapiens | |
| 0.00067 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 10.0 | Homo sapiens | |
| 0.0013 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 9.0 | Homo sapiens | |
| 0.0033 | - |
sulfite | mutant Y343N/R472m, 25°C, pH 8.5 | Homo sapiens | |
| 0.0047 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.5 | Homo sapiens | |
| 0.0049 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 10.0 | Homo sapiens | |
| 0.0069 | - |
sulfite | mutant Y343N/R472m, 25°C, pH 8.0 | Homo sapiens | |
| 0.008 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 6.0 | Homo sapiens | |
| 0.01 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 9.0 | Homo sapiens | |
| 0.0124 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.5 | Homo sapiens | |
| 0.0244 | - |
sulfite | mutant Y343N/R472M, 25°C, pH 7.0 | Homo sapiens | |
| 0.0279 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.5 | Homo sapiens | |
| 0.036 | - |
sulfite | mutant Y343N, 25°C, pH 10.0 | Homo sapiens | |
| 0.069 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 9.0 | Homo sapiens | |
| 0.0866 | - |
sulfite | mutant Y343N, 25°C, pH 9.5 | Homo sapiens | |
| 0.167 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 8.0 | Homo sapiens | |
| 0.173 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 6.0 | Homo sapiens | |
| 0.214 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 8.5 | Gallus gallus | |
| 0.237 | - |
sulfite | mutant Y343N/R472M/V474M, 25°C, pH 7.0 | Homo sapiens | |
| 0.316 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.5 | Homo sapiens | |
| 0.632 | - |
sulfite | mutant Y343N, 25°C, pH 9.0 | Homo sapiens | |
| 0.725 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 8.0 | Homo sapiens | |
| 1.97 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 7.0 | Homo sapiens | |
| 1.99 | - |
sulfite | mutant Y343N, 25°C, pH 8.5 | Homo sapiens | |
| 2.35 | - |
sulfite | mutant Y343F/R472Q, 25°C, pH 6.0 | Homo sapiens | |
| 3.33 | - |
sulfite | mutant Y343N, 25°C, pH 6.0 | Homo sapiens | |
| 4.63 | - |
sulfite | mutant Y343N, 25°C, pH 8.0 | Homo sapiens | |
| 8.6 | - |
sulfite | wild-type, 25°C, pH 7.0 | Gallus gallus | |
| 13.5 | - |
sulfite | mutant Y343N, 25°C, pH 7.0 | Homo sapiens | |
| 30.8 | - |
sulfite | mutant V474M, 25°C, pH 10.0 | Homo sapiens | |
| 53.6 | - |
sulfite | mutant V474M, 25°C, pH 9.5 | Homo sapiens | |
| 136 | - |
sulfite | mutant V474M, 25°C, pH 9.0 | Homo sapiens | |
| 246 | - |
sulfite | wild-type, 25°C, pH 10.0 | Homo sapiens | |
| 392 | - |
sulfite | wild-type, 25°C, pH 9.5 | Homo sapiens | |
| 446 | - |
sulfite | mutant V474M, 25°C, pH 8.5 | Homo sapiens | |
| 558 | - |
sulfite | mutant V474M, 25°C, pH 8.0 | Homo sapiens | |
| 851 | - |
sulfite | mutant V474M, 25°C, pH 7.0 | Homo sapiens | |
| 1160 | - |
sulfite | wild-type, 25°C, pH 9.0 | Homo sapiens | |
| 1300 | - |
sulfite | mutant V474M, 25°C, pH 6.0 | Homo sapiens | |
| 3260 | - |
sulfite | wild-type, 25°C, pH 8.5 | Homo sapiens | |
| 5950 | - |
sulfite | wild-type, 25°C, pH 8.0 | Homo sapiens | |
| 8690 | - |
sulfite | wild-type, 25°C, pH 8.5 | Gallus gallus | |
| 8900 | - |
sulfite | wild-type, 25°C, pH 7.0 | Homo sapiens | |
| 10000 | - |
sulfite | wild-type, 25°C, pH 6.0 | Homo sapiens | |
| 27000 | - |
sulfite | mutant Y322N/R450M, 25°C, pH 7.0 | Gallus gallus | |
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