Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain TP1000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G473A | mutant is able to dimerize and has steady-state activity comparable to that of the wild type, stopped-flow analysis of the reductive half-reaction of this variant yields a rate constant nearly 3 times higher than that of the wild type | Homo sapiens |
G473D | monomer, mutant is severely impaired both in the ability to bind sulfite and in catalysis, with a second-order rate constant 5 orders of magnitude lower than that of the wild type, significant random-coil formation | Homo sapiens |
G473W | monomer, mutant with 5fold higher activity than G473D and nearly wild-type activity at pH 7.0 when ferricyanide is the electron acceptor, significant random-coil formation | Homo sapiens |
R212A/G473D | mutant is able to oligomerize but has undetectable activity, significant random-coil formation | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00043 | - |
cytochrome c | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
0.00082 | - |
cytochrome c | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
0.00123 | - |
cytochrome c | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
0.00129 | - |
sulfite | wild type enzyme, pH 6.0 | Homo sapiens | |
0.00141 | - |
cytochrome c | wild type enzyme, pH 7.0 | Homo sapiens | |
0.00162 | - |
sulfite | wild type enzyme, pH 6.5 | Homo sapiens | |
0.00173 | - |
cytochrome c | wild type enzyme, pH 6.0 | Homo sapiens | |
0.00272 | - |
sulfite | wild type enzyme, pH 7.0 | Homo sapiens | |
0.00339 | - |
sulfite | wild type enzyme, pH 7.5 | Homo sapiens | |
0.00371 | - |
cytochrome c | mutant enzyme G473D, pH 8.6 | Homo sapiens | |
0.00435 | - |
sulfite | wild type enzyme, pH 8.0 | Homo sapiens | |
0.0044 | - |
cytochrome c | wild type enzyme, pH 8.5 | Homo sapiens | |
0.00453 | - |
sulfite | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
0.00474 | - |
cytochrome c | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
0.00536 | - |
sulfite | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
0.00536 | - |
cytochrome c | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
0.00825 | - |
sulfite | wild type enzyme, pH 8.5 | Homo sapiens | |
0.014 | - |
cytochrome c | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
0.0172 | - |
sulfite | mutant enzyme G473A, pH 7.4 | Homo sapiens | |
0.0221 | - |
sulfite | wild type enzyme, pH 9.0 | Homo sapiens | |
0.0357 | - |
cytochrome c | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
0.0362 | - |
cytochrome c | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
0.0487 | - |
sulfite | mutant enzyme G473A, pH 8.0 | Homo sapiens | |
0.0635 | - |
cytochrome c | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
0.0671 | - |
sulfite | wild type enzyme, pH 9.5 | Homo sapiens | |
0.0905 | - |
cytochrome c | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
0.107 | - |
sulfite | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
0.107 | - |
cytochrome c | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
0.33 | - |
sulfite | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
0.623 | - |
sulfite | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
0.774 | - |
sulfite | mutant enzyme G473A, pH 9.1 | Homo sapiens | |
0.99 | - |
sulfite | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
1.063 | - |
sulfite | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
1.223 | - |
sulfite | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
1.66 | - |
sulfite | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
1.91 | - |
sulfite | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
2.034 | - |
sulfite | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
2.04 | - |
sulfite | mutant enzyme G473D, pH 8.5 | Homo sapiens | |
3.684 | - |
sulfite | mutant enzyme G473A, pH 10.0 | Homo sapiens | |
10.41 | - |
sulfite | mutant enzyme G473W, pH 9.0 | Homo sapiens | |
25.88 | - |
sulfite | mutant enzyme G473D, pH 9.1 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | consists of a single molybdenum atom coordinated through the dithiolene group of a single molybdopterin molecule | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
52000 | - |
2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
53500 | - |
1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
54500 | - |
1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
66600 | - |
1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
101000 | - |
wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography and Superdex 200 16/60 FPLC column gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + cytochrome c | - |
Homo sapiens | sulfate + reduced cytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, wild type enzyme, sedimentation equilibrium analysis | Homo sapiens |
monomer | 1 * 52000, mutant G473D, calculation from amino acid sequence | Homo sapiens |
monomer | 1 * 53500, mutant G473D, gel filtration and sedimentation equilibrium analysis | Homo sapiens |
monomer | 1 * 54500, mutant G473W, sedimentation equilibrium analysis | Homo sapiens |
monomer | 1 * 66600, double mutant R212A/G473D, sedimentation equilibrium analysis | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
sulfite | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
0.28 | - |
sulfite | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
0.34 | - |
cytochrome c | mutant enzyme G473D, pH 6.0 | Homo sapiens | |
0.42 | - |
sulfite | mutant enzyme G473D, pH 9.1 | Homo sapiens | |
0.48 | - |
cytochrome c | mutant enzyme G473D, pH 8.6 | Homo sapiens | |
0.5 | - |
sulfite | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
0.53 | - |
cytochrome c | mutant enzyme G473D, pH 6.5 | Homo sapiens | |
0.54 | - |
sulfite | mutant enzyme G473D, pH 8.5 | Homo sapiens | |
0.57 | - |
sulfite | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
0.58 | - |
sulfite | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
0.59 | - |
cytochrome c | mutant enzyme G473D, pH 8.0 | Homo sapiens | |
0.6 | - |
sulfite | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
0.62 | - |
cytochrome c | mutant enzyme G473D, pH 7.5 | Homo sapiens | |
0.78 | - |
cytochrome c | mutant enzyme G473D, pH 7.0 | Homo sapiens | |
1.35 | - |
sulfite | mutant enzyme G473W, pH 9.0 | Homo sapiens | |
1.67 | - |
cytochrome c | mutant enzyme G473W, pH 6.0 | Homo sapiens | |
1.8 | - |
sulfite | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
1.95 | - |
cytochrome c | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
2.48 | - |
sulfite | mutant enzyme G473W, pH 8.5 | Homo sapiens | |
2.94 | - |
cytochrome c | mutant enzyme G473W, pH 7.0 | Homo sapiens | |
3.83 | - |
cytochrome c | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
4.15 | - |
sulfite | mutant enzyme G473A, pH 6.0 | Homo sapiens | |
12.4 | - |
cytochrome c | wild type enzyme, pH 6.0 | Homo sapiens | |
12.8 | - |
cytochrome c | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
13.2 | - |
sulfite | wild type enzyme, pH 6.0 | Homo sapiens | |
15.1 | - |
sulfite | mutant enzyme G473A, pH 10.0 | Homo sapiens | |
15.9 | - |
sulfite | mutant enzyme G473A, pH 7.0 | Homo sapiens | |
17.7 | - |
sulfite | wild type enzyme, pH 6.5 | Homo sapiens | |
18.1 | - |
cytochrome c | wild type enzyme, pH 7.0 | Homo sapiens | |
21.6 | - |
sulfite | mutant enzyme G473A, pH 7.4 | Homo sapiens | |
24.2 | - |
sulfite | wild type enzyme, pH 7.0 | Homo sapiens | |
24.7 | - |
sulfite | wild type enzyme, pH 7.5 | Homo sapiens | |
25.4 | - |
cytochrome c | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
25.7 | - |
sulfite | wild type enzyme, pH 9.0 | Homo sapiens | |
25.9 | - |
sulfite | wild type enzyme, pH 8.0 | Homo sapiens | |
26.2 | - |
sulfite | mutant enzyme G473A, pH 8.0 | Homo sapiens | |
26.3 | - |
sulfite | wild type enzyme, pH 9.5 | Homo sapiens | |
26.9 | - |
cytochrome c | wild type enzyme, pH 8.5 | Homo sapiens | |
26.9 | - |
sulfite | wild type enzyme, pH 8.5 | Homo sapiens | |
28.4 | - |
sulfite | mutant enzyme G473A, pH 8.5 | Homo sapiens | |
31.9 | - |
sulfite | mutant enzyme G473A, pH 9.1 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Homo sapiens |