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Literature summary for 1.8.3.1 extracted from

  • Feng, C.; Wilson, H.L.; Tollin, G.; Astashkin, A.V.; Hazzard, J.T.; Rajagopalan, K.V.; Enemark, J.H.
    The pathogenic human sulfite oxidase mutants G473D and A208D are defective in intramolecular electron transfer (2005), Biochemistry, 44, 13734-13743.
    View publication on PubMed
Search for more literature for 1.8.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain TP1000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
A208D the intramolecular electron transfer rate constants at pH 6.0 are decreased by 3 orders of magnitude relative to that of the wild type, the active site structure of the Mo(V) form of A208D is different from that of the wild type Homo sapiens
G473A dimer Homo sapiens
G473D monomer, the Mo(V) active site structure is similar to that of the wild type, and the IET rate constant is only 2.6fold smaller than that of the wild type Homo sapiens
G473D/R212A shows no intramolecular electron transfer rate Homo sapiens
G473W monomer Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00129
-
 sulfite wild type enzyme in 20 mM Tris at pH 6.0 Homo sapiens
0.00453
-
 sulfite mutant enzyme G473A in 20 mM Tris at pH 6.0 Homo sapiens
0.00825
-
 sulfite wild type enzyme in 20 mM Tris at pH 8.5 Homo sapiens
0.0692
-
 sulfite mutant enzyme A208D in 20 mM Tris at pH 6.0 Homo sapiens
0.107
-
 sulfite mutant enzyme G473A in 20 mM Tris at pH 8.5 Homo sapiens
1.39
-
 sulfite mutant enzyme A208D in 20 mM Tris at pH 8.5 Homo sapiens
1.66
-
 sulfite mutant enzyme G473D in 20 mM Tris at pH 6.0 Homo sapiens
1.91
-
 sulfite mutant enzyme G473W in 20 mM Tris at pH 6.0 Homo sapiens
2.03
-
 sulfite mutant enzyme G473W in 20 mM Tris at pH 8.5 Homo sapiens
2.04
-
 sulfite mutant enzyme G473D in 20 mM Tris at pH 8.5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 200 FPLC column gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + H2O + A
-
 Homo sapiens SO42- + AH2
-
 ?

Subunits

Subunits Comment Organism
dimer wild type enzyme, mutant enzymes G473A and A208D Homo sapiens
monomer mutant enzymes G473D and G473W Homo sapiens

Synonyms

Synonyms Comment Organism
SO
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 sulfite mutant enzyme G473D in 20 mM Tris at pH 6.0 Homo sapiens
0.15
-
 sulfite mutant enzyme A208D in 20 mM Tris at pH 6.0 Homo sapiens
0.54
-
 sulfite mutant enzyme G473D in 20 mM Tris at pH 8.5 Homo sapiens
0.6
-
 sulfite mutant enzyme G473W in 20 mM Tris at pH 6.0 Homo sapiens
0.75
-
 sulfite mutant enzyme A208D in 20 mM Tris at pH 8.5 Homo sapiens
2.48
-
 sulfite mutant enzyme G473W in 20 mM Tris at pH 8.5 Homo sapiens
4.15
-
 sulfite mutant enzyme G473A in 20 mM Tris at pH 6.0 Homo sapiens
13.2
-
 sulfite wild type enzyme in 20 mM Tris at pH 6.0 Homo sapiens
26.9
-
 sulfite wild type enzyme in 20 mM Tris at pH 8.5 Homo sapiens
28.4
-
 sulfite mutant enzyme G473A in 20 mM Tris at pH 8.5 Homo sapiens