Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Thermithiobacillus tepidarius | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme | Acidiphilium acidophilum |
24000 | - |
2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme | Acidiphilium acidophilum |
45000 | - |
3 * 45000, SDS-PAGE | Thermithiobacillus tepidarius |
102000 | - |
- |
Acidiphilium acidophilum |
138000 | - |
- |
Thermithiobacillus tepidarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidiphilium acidophilum | - |
- |
- |
Halothiobacillus neapolitanus | - |
- |
- |
Thermithiobacillus tepidarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using ion exchange chromatography, gel filtration and hydrophobic interaction chromatography | Thermithiobacillus tepidarius |
using ammonium sulfate precipitation, hydrophobic interaction chromatography, anion-exchange chromatography and gel filtration | Acidiphilium acidophilum |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 24000 + 2 * 20000, SDS-PAGE, both subunits contain c553-type heme | Acidiphilium acidophilum |
trimer | 3 * 45000, SDS-PAGE | Thermithiobacillus tepidarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Halothiobacillus neapolitanus |
4.5 | - |
- |
Thermithiobacillus tepidarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | 5.3 mole per mol of native enzyme, made up of two different c-553 hemes, present in both subunit types | Acidiphilium acidophilum |