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Literature summary for 1.8.2.2 extracted from

  • Lyric, R.M.; Suzuki, I.
    Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. 3. Properties of thiosulfate-oxidizing enzyme and proposed pathway of thiosulfate oxidation (1970), Can. J. Biochem., 48, 355-363.
    View publication on PubMed
Search for more literature for 1.8.2.2

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl 50% inhibition at 1 mM, a 60 min incubation period is necessary to potentiate the inhibition Thiobacillus thioparus
EDTA 44% inhibition at 10 mM, a 15 min incubation period is necessary to potentiate the inhibition Thiobacillus thioparus
HgCl2 100% inhibition at 0.5 mM Thiobacillus thioparus
N-ethylmaleimide 100% inhibition at 1 mM, a 15 min incubation period is necessary to potentiate the inhibition Thiobacillus thioparus
p-chloromercuribenzoate 72.8% inhibition at 1 mM Thiobacillus thioparus
SO32- 50% inhibition at 0.005 mM , time-dependent inhibition, irreversible Thiobacillus thioparus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Thiobacillus thioparus
0.005
-
 thiosulfate cytochrome c as electron acceptor Thiobacillus thioparus
0.1
-
 thiosulfate ferricyanide as electron acceptor Thiobacillus thioparus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains 2 mol of non-heme iron Thiobacillus thioparus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
 sucrose density gradient method Thiobacillus thioparus

Organism

Organism UniProt Comment Textmining
Thiobacillus thioparus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using sonication, column chromatography on DEAE-cellulose, pH-adjustment to 4.5 and calcium phosphate gel treatment Thiobacillus thioparus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
146.1
-
-
 Thiobacillus thioparus

Storage Stability

Storage Stability Organism
-20°C, the ability of the enzyme to couple with cytochrome c decreases during storage Thiobacillus thioparus
4°C, 10% loss of activity after 6 h and 50% loss of activity after 5 days Thiobacillus thioparus
frozen, stable, indefinitely as measured by ferricyanide reduction Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NAD+, methylene blue, 2,6-dichlorophenol indophenol Thiobacillus thioparus ?
-
 ?
additional information no activity with: NADP+, O2 Thiobacillus thioparus ?
-
 ?
thiosulfate + oxidized acceptor cytochrome c of yeast is a poor electron acceptor Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor horse cytochrome c is a poor electron acceptor Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor ferricyanide is a much better electron acceptor than cytochrome c Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?
thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 6 h, 10% loss of activity, 50% loss of activity after 5 days Thiobacillus thioparus
55
-
 5 min, stable Thiobacillus thioparus
60
-
 5 min, 50% loss of activity Thiobacillus thioparus
100
-
 1 min, complete loss of activity Thiobacillus thioparus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 below, using ferricyanide as electron acceptor Thiobacillus thioparus
6.5
-
 broad, with cytochrome c as electron acceptor Thiobacillus thioparus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.8 little change in activity between Thiobacillus thioparus