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Literature summary for 1.8.2.1 extracted from

  • Ogawa, T.; Furusawa, T.; Shiga, M.; Seo, D.; Sakurai, H.; Inoue, K.
    Biochemical studies of a soxF-encoded monomeric flavoprotein purified from the green sulfur bacterium Chlorobaculum tepidum that stimulates in vitro thiosulfate oxidation (2010), Biosci. Biotechnol. Biochem., 74, 771-780.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
ferricytochrome c pH 7.8, 25°C Chlorobaculum tepidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
1 * 42000, SDS-PAGE, 1 * 43770, MALDI-TOF Chlorobaculum tepidum
43770
-
1 * 42000, SDS-PAGE, 1 * 43770, MALDI-TOF Chlorobaculum tepidum
48000
-
gel filtration Chlorobaculum tepidum

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KDN1
-
-

Purification (Commentary)

Purification (Comment) Organism
protein is yellow in colour Chlorobaculum tepidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + 2 ferricytochrome c + H2O in the absence of the core thiosulfate oxidizing multi-enzyme system, enzyme catalyzes sulfide-dependent reduction of Chlorobaculum tepidum cytochrome c-554 Chlorobaculum tepidum sulfate + 2 ferrocytochrome c + 2 H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE, 1 * 43770, MALDI-TOF Chlorobaculum tepidum

Synonyms

Synonyms Comment Organism
CT1015
-
Chlorobaculum tepidum
SoxF
-
Chlorobaculum tepidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
FAD enzyme covalently binds one FAD Chlorobaculum tepidum

General Information

General Information Comment Organism
physiological function enzyme enhances the thiosulfate oxidation activity of the core thiosulfate oxidizing multi-enzyme system TOMES with various cytochromes as electron acceptors, enzyme mediates electron transfer between the components of core TOMES and externally added cytochromes Chlorobaculum tepidum