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Literature summary for 1.8.2.1 extracted from

  • Bailey, S.; Rapson, T.; Johnson-Winters, K.; Astashkin, A.V.; Enemark, J.H.; Kappler, U.
    Molecular basis for enzymatic sulfite oxidation: how three conserved active site residues shape enzyme activity (2009), J. Biol. Chem., 284, 2053-2063.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.2.1

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure Starkeya novella

Protein Variants

Protein Variants Comment Organism
H57A mutant with reduced activity, Tyr-236 and His-57 are necessary to stabilize Arg-55 in a position for optimal hydrogen bonding to the heme 6-propionate Starkeya novella
R55M mutant with reduced activity, R-55 is an important position close to the substrate binding site, where it makes hydrogen bonds to the equatorial oxo ligand of the molybdenum, to Gln-33, and a nearby water molecule. It also forms a salt bridge, comprising two hydrogen bonds, with propionate-6 of the heme moiety of the cytochrome subunit Starkeya novella
Y236F mutant with reduced activity, Tyr-236 and His-57 are necessary to stabilize Arg-55 in a position for optimal hydrogen bonding to the heme 6-propionate Starkeya novella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000004
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F Starkeya novella
0.000007
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F Starkeya novella
0.0006
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme Starkeya novella
0.0011
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme Starkeya novella
0.0037
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme Starkeya novella
0.0071
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme Starkeya novella
0.022
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme Starkeya novella
0.026
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F Starkeya novella
0.042
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F Starkeya novella
0.086
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme Starkeya novella
0.114
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F Starkeya novella
0.189
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A Starkeya novella
0.22
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A Starkeya novella
0.27
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A Starkeya novella
0.29
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A Starkeya novella
0.324
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme Starkeya novella
0.332
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236Fyme Starkeya novella
0.452
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A Starkeya novella
0.667
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A Starkeya novella
0.812
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M Starkeya novella
1.087
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M Starkeya novella
1.155
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F Starkeya novella
1.46
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A Starkeya novella
1.63
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M Starkeya novella
1.66
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme Starkeya novella
1.95
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M Starkeya novella
3.39
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme Starkeya novella
3.62
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M Starkeya novella
4.456
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F Starkeya novella
8.17
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M Starkeya novella
12.2
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A Starkeya novella
15.49
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F Starkeya novella

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum
-
 Starkeya novella

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfite + ferricytochrome c + H2O Starkeya novella
-
 sulfate + ferrocytochrome c + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Starkeya novella Q9LA16
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + ferricytochrome c + H2O
-
 Starkeya novella sulfate + ferrocytochrome c + H+
-
 ?
sulfite + ferricytochrome c + H2O cytochrome c from horse heart Starkeya novella sulfate + ferrocytochrome c + H+
-
 ?

Synonyms

Synonyms Comment Organism
SDH
-
 Starkeya novella

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 3.7 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme Starkeya novella
36.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F Starkeya novella
41.6
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F Starkeya novella
48.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F Starkeya novella
51.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F Starkeya novella
52.7
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F Starkeya novella
53.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F Starkeya novella
58.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236F Starkeya novella
63.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme Starkeya novella
64
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M Starkeya novella
64
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M Starkeya novella
64
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F Starkeya novella
66.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M Starkeya novella
68.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M Starkeya novella
70.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M Starkeya novella
73.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M Starkeya novella
86.2
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme Starkeya novella
88.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F Starkeya novella
148.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A Starkeya novella
158.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme Starkeya novella
192.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A Starkeya novella
214.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A Starkeya novella
214.6
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A Starkeya novella
220.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A Starkeya novella
222.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A Starkeya novella
226.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A Starkeya novella
238.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A Starkeya novella
293.4
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme Starkeya novella
345.3
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme Starkeya novella
410
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme Starkeya novella
431
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme Starkeya novella
519
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme Starkeya novella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Starkeya novella

pH Range

pH Minimum pH Maximum Comment Organism
8 10 less than 50% of maximal activity above and below Starkeya novella

Cofactor

Cofactor Comment Organism Structure
heme c
-
 Starkeya novella
molybdenum cofactor (molybdopterin-S,S)-oxo-molybdenum Starkeya novella

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.84
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, mutant Y236F Starkeya novella
8.98
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.9, mutant R55M Starkeya novella
11.6
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant Y236F Starkeya novella
18.9
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant R55M Starkeya novella
34
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant R55M Starkeya novella
54.5
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant Y236F Starkeya novella
58.9
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.2, mutant R55M Starkeya novella
67.7
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 10.0, wild-type enzyme Starkeya novella
86.8
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 5.8, mutant R55M Starkeya novella
122
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, mutant H57A Starkeya novella
132
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, mutant H57A Starkeya novella
173
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant Y236F Starkeya novella
251
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.5, wild-type enzyme Starkeya novella
358
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant H57A Starkeya novella
461
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant Y236F Starkeya novella
492
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, mutant H57A Starkeya novella
782
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant H57A Starkeya novella
799
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, mutant H57A Starkeya novella
1010
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant H57A Starkeya novella
1130
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant H57A Starkeya novella
1140
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, mutant Y236F Starkeya novella
1500
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 9.0, wild-type enzyme Starkeya novella
1970
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, mutant Y236F Starkeya novella
3930
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.6, mutant R55M Starkeya novella
4880
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.5, wild-type enzyme Starkeya novella
5600
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, mutant Y236F Starkeya novella
8420
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, mutant Y236F Starkeya novella
15300
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 8.0, wild-type enzyme Starkeya novella
40300
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.5, wild-type enzyme Starkeya novella
42700
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 7.0, wild-type enzyme Starkeya novella
75500
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.5, wild-type enzyme Starkeya novella
106000
-
 sulfite 25°C, 20 mM Tris acetate buffer, pH 6.0, wild-type enzyme Starkeya novella