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Literature summary for 1.8.2.1 extracted from

  • Rapson, T.D.; Kappler, U.; Bernhardt, P.V.
    Direct catalytic electrochemistry of sulfite dehydrogenase: Mechanistic insights and contrasts with related Mo enzymes (2008), Biochim. Biophys. Acta, 1777, 1319-1325.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information SDH emerges at saturating substrate conditions and occurs at a potential of ca. +320 mV vs normal hydrogen electrode Starkeya novella

Inhibitors

Inhibitors Comment Organism Structure
Cl- 30% electrochemical activity at 100 mM inhibitor concentration, at 50 mM 50% inhibition Starkeya novella
HPO42- 18% electrochemical activity at 100 mM inhibitor concentration, at 20 mM 50% inhibition Starkeya novella
NO3- 37% electrochemical activity at 100 mM inhibitor concentration, at 3 mM 50% inhibition Starkeya novella
SO42- 19% electrochemical activity at 100 mM inhibitor concentration, at 15 mM 50% inhibition Starkeya novella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
sulfite at pH 7.7 and 294 mV Starkeya novella
0.0125
-
sulfite at pH 7.7 and 360 mV Starkeya novella
0.054
-
sulfite at pH 8.0 and 345 mV Starkeya novella
0.063
-
sulfite at pH 8.0 and 264 mV Starkeya novella
0.269
-
sulfite at pH 8.5 and 345 mV Starkeya novella
0.283
-
sulfite at pH 8.5 and 283 mV Starkeya novella

Metals/Ions

Metals/Ions Comment Organism Structure
Mo does not play a rate defining role in the catalytic mechanism of SDH before turnover Starkeya novella

Organism

Organism UniProt Comment Textmining
Starkeya novella
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified from a heterologous expression system in Rhodobacter capsulatus Starkeya novella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + H2O + acceptor
-
Starkeya novella sulfate + reduced acceptor + 2 H+
-
?

Synonyms

Synonyms Comment Organism
SDH
-
Starkeya novella
SorAB
-
Starkeya novella