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Literature summary for 1.8.2.1 extracted from
- Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. I. Survey of enzymes and properties of sulfite: cytochrome c oxidoreductase (1970), Can. J. Biochem., 48, 334-343.
General Stability
| General Stability | Organism |
|---|---|
| thawing and refreezing has no effect | Thiobacillus thioparus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 5 mM, complete inhibition | Thiobacillus thioparus |  |
| 2,2'-bipyridyl | 5 mM, complete inhibition | Thiobacillus thioparus | |
| AMP | - |
Thiobacillus thioparus | |
| arsenite | 1 mM, 83% inhibition | Thiobacillus thioparus | |
| Cl- | non-competitive | Thiobacillus thioparus | |
| EDTA | 4 mM complete inhibition | Thiobacillus thioparus | |
| HgCl2 | 10 mM, complete inhibition | Thiobacillus thioparus | |
| KCl | - |
Thiobacillus thioparus | |
| NaCl | noncompetitive | Thiobacillus thioparus | |
| NEM | 1 mM, 32% inhibition | Thiobacillus thioparus | |
| PCMB | 1 mM, complete inhibition | Thiobacillus thioparus | |
| phosphate | 50 mM, 90% inhibition, uncompetitive with respect to sulfite | Thiobacillus thioparus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
ferricytochrome c | - |
Thiobacillus thioparus | |
| 0.088 | - |
sulfite | - |
Thiobacillus thioparus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | enzyme contains nonheme iron | Thiobacillus thioparus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
sucrose density gradient method | Thiobacillus thioparus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thiobacillus thioparus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thiobacillus thioparus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.69 | - |
- |
Thiobacillus thioparus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several months | Thiobacillus thioparus |
| 4°C, 10-20% loss of activity after 6 h at room temperature, 80% loss of activity after 5 days | Thiobacillus thioparus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfite + ferricyanide + H2O | - |
Thiobacillus thioparus | sulfate + ferrocyanide + H+ | - |
? | |
| sulfite + ferricytochrome c + H2O | horse heart cytochrome or yeast cytochrome | Thiobacillus thioparus | sulfate + ferrocytochrome c + H+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
9 min, 95% loss of activity | Thiobacillus thioparus |
| 60 | - |
6 min, 95% loss of activity | Thiobacillus thioparus |
| 100 | - |
1 min, complete loss of activity | Thiobacillus thioparus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
with Tricine-HCl or Tricine-NaOH buffer | Thiobacillus thioparus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38 | - |
Cl- | - |
Thiobacillus thioparus | |
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