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Literature summary for 1.8.2.1 extracted from

  • Toghrol, F.; Southerland, W.M.
    Purification of Thiobacillus novellus sulfite oxidase. Evidence for the presence of heme and molybdenum (1983), J. Biol. Chem., 258, 6762-6766.
    View publication on PubMed

General Stability

General Stability Organism
proteolytic treatment with trypsin at room temperature for up to 5 h at trypsin to enzyme ratios of 1/20 and 1/1 does not affect the activity of the enzyme Starkeya novella

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum enzyme contains molybdenum Starkeya novella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration Starkeya novella

Organism

Organism UniProt Comment Textmining
Starkeya novella
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Starkeya novella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Starkeya novella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfite + ferricyanide + H2O 3-5% of the activity with ferricytochrome Starkeya novella sulfate + ferrocyanide + H+
-
?
sulfite + ferricytochrome c + H2O
-
Starkeya novella sulfate + ferrocytochrome c + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Starkeya novella

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
rapid inactivation of oxidized enzyme, substrate-reduced enzyme remains active for more than 1 h Starkeya novella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Starkeya novella

Cofactor

Cofactor Comment Organism Structure
heme enzyme contains heme Starkeya novella