Inhibitors | Comment | Organism | Structure |
---|---|---|---|
auranofin | complete inhibition at 0.7 nM | Taenia crassiceps |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00087 | - |
thioredoxin disulfide | at pH 7.8 and 25°C | Taenia crassiceps | |
0.019 | - |
NADPH | at pH 7.8 and 25°C | Taenia crassiceps | |
0.0345 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | at pH 7.8 and 25°C | Taenia crassiceps | |
0.041 | - |
GSSG | at pH 7.8 and 25°C | Taenia crassiceps |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Taenia crassiceps | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Taenia crassiceps |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH + H+ | Taenia crassiceps | - |
GSH + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | Taenia crassiceps | - |
thioredoxin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Taenia crassiceps | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sephacel gel filtration, hydroxyapatite chromatography column chromatography, and Cibacron blue chromatography column chromatography | Taenia crassiceps |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Taenia crassiceps | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.7 | - |
crude extract, using GSSG as substrate, at pH 7.8 and 25°C | Taenia crassiceps |
35 | - |
crude extract, using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.8 and 25°C | Taenia crassiceps |
128.5 | - |
crude extract, using hydroxyethyl disulfide as substrate, at pH 7.8 and 25°C | Taenia crassiceps |
229 | - |
after 31fold purification, using GSSG as substrate, at pH 7.8 and 25°C | Taenia crassiceps |
1091 | - |
after 31fold purification, using 5,5'-dithiobis(2-nitrobenzoic acid) as substrate, at pH 7.8 and 25°C | Taenia crassiceps |
2243 | - |
after 31fold purification, using hydroxyethyl disulfide as substrate, at pH 7.8 and 25°C | Taenia crassiceps |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, 15 days, remains stable | Taenia crassiceps |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Taenia crassiceps | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
GSSG + NADPH + H+ | - |
Taenia crassiceps | GSH + NADP+ | - |
? | |
hydroxyethyl disulfide + NADPH + H+ | - |
Taenia crassiceps | ? | - |
? | |
additional information | low catalytic efficiency with H2O2 | Taenia crassiceps | ? | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Taenia crassiceps | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Taenia crassiceps |
Synonyms | Comment | Organism |
---|---|---|
TGR | - |
Taenia crassiceps |
thioredoxin-glutathione reductase | - |
Taenia crassiceps |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
for 5,5'-dithiobis(2-nitrobenzoic acid) reductase activity | Taenia crassiceps |
40 | - |
for glutathione reductase activity | Taenia crassiceps |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | at pH 7.8 and 25°C | Taenia crassiceps | |
0.47 | - |
thioredoxin disulfide | at pH 7.8 and 25°C | Taenia crassiceps | |
0.84 | - |
GSSG | at pH 7.8 and 25°C | Taenia crassiceps |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
for 5,5'-dithiobis(2-nitrobenzoic acid) reductase activity | Taenia crassiceps |
7.8 | - |
for glutathione reductase activity | Taenia crassiceps |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Taenia crassiceps | |
NADPH | - |
Taenia crassiceps |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is able to protect glutamine synthetase from oxidative inactivation, suggesting that the enzyme is competent to contend with oxidative stress | Taenia crassiceps |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.7 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | at pH 7.8 and 25°C | Taenia crassiceps | |
22 | - |
GSSG | at pH 7.8 and 25°C | Taenia crassiceps | |
540 | - |
thioredoxin disulfide | at pH 7.8 and 25°C | Taenia crassiceps |