Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Echinococcus granulosus |
Protein Variants | Comment | Organism |
---|---|---|
C31S | the mutant has negligible deglutathionylase and GR activities when compared with wild type enzyme | Echinococcus granulosus |
C34S | both the deglutathionylase and GR activities of the mutant are only slightly lower when compared with wild type enzyme | Echinococcus granulosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NOC-7 | i.e. 1-hydroxy-2-oxo-3-(N-3-methyl-aminopropyl)-3-methyl-1-triazene, complete inhibition at 0.1 mM | Echinococcus granulosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH + H+ | Echinococcus granulosus | - |
GSH + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | Echinococcus granulosus | - |
thioredoxin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Echinococcus granulosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Echinococcus granulosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Echinococcus granulosus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
GSSG + NADPH + H+ | - |
Echinococcus granulosus | GSH + NADP+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | - |
Echinococcus granulosus | thioredoxin + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TGR | - |
Echinococcus granulosus |
thioredoxin-glutathione reductase | - |
Echinococcus granulosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Echinococcus granulosus | |
NADPH | - |
Echinococcus granulosus |