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Literature summary for 1.8.1.B1 extracted from

  • Lea, W.A.; Jadhav, A.; Rai, G.; Sayed, A.A.; Cass, C.L.; Inglese, J.; Williams, D.L.; Austin, C.P.; Simeonov, A.
    A 1,536-well-based kinetic HTS assay for inhibitors of Schistosoma mansoni thioredoxin glutathione reductase (2008), Assay Drug Dev. Technol., 6, 551-555.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.B1

Application

Application Comment Organism
medicine the enzyme presents a target for anti-schistosomiasis drug development Schistosoma mansoni

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme with a fused bacterial-type SECIS element is expressed in Escherichia coli strain BL21(DE3) Schistosoma mansoni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Schistosoma mansoni the parasite-specific enzyme thioredoxin glutathione reductase is a component of the defense system ?
-
 ?

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1536-well based kinetic absorbance assay for thioredoxin glutathione reductase. The assay uses the catalytic reduction of DTNB by NADPH in the presence of thioredoxin glutathione reductase Schistosoma mansoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
 Schistosoma mansoni ?
-
 ?
additional information the parasite-specific enzyme thioredoxin glutathione reductase is a component of the defense system Schistosoma mansoni ?
-
 ?

Synonyms

Synonyms Comment Organism
TGR
-
 Schistosoma mansoni
thioredoxin glutathione reductase
-
 Schistosoma mansoni