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Literature summary for 1.8.1.9 extracted from

  • Javvadi, P.; Hertan, L.; Kosoff, R.; Datta, T.; Kolev, J.; Mick, R.; Tuttle, S.W.; Koumenis, C.
    Thioredoxin reductase-1 mediates curcumin-induced radiosensitization of squamous carcinoma cells (2010), Cancer Res., 70, 1941-1950.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.8.1.9

Inhibitors

Inhibitors Comment Organism Structure
additional information tetrahydrocurcumin has no significant effect on TxnRd activity at doses of up to 0.05 mM Homo sapiens
trans,trans-curcumin
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
FaDu cell
-
 Homo sapiens
-
HEK-293 cell HEK-293 cells show low basal levels of TxnRd1 Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
keratinocyte
-
 Homo sapiens
-
MSK-Leuk1 cell
-
 Homo sapiens
-
SiHa cell
-
 Homo sapiens
-
squamous cell carcinoma cell
-
 Homo sapiens
-
UM-SCC-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Homo sapiens 2-nitro-5-thiobenzoate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
thioredoxin reductase
-
 Homo sapiens
Txnrd1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens
NADPH
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.01
-
 pH and temperature not specified in the publication Homo sapiens trans,trans-curcumin

Expression

Organism Comment Expression
Homo sapiens TxnRd1 expression is elevated by more than 50fold in FaDu and HeLa cells and by more than 20fold in SCC-1 cells compared with either MSK-Leuk1 cells or keratinocytes. TxnRd activity is about 20fold higher in FaDu and HeLa cells and about 7fold higher in SCC-1 cells compared with that in keratinocytes or MSK-Leuk1 cells up

General Information

General Information Comment Organism
malfunction stable knockdown of TxnRd1 in both HeLa and FaDu cells nearly abolishes curcumin-mediated radiosensitization. TxnRd1 knockdown cells show decreased radiation-induced reactive oxygen species and sustained extracellular signal-regulated kinase 1/2 activation Homo sapiens
physiological function thioredoxin reductase-1 mediates curcumin-induced radiosensitization of squamous carcinoma cells. Overexpressing catalytically active TxnRd1 in HEK-293 cells, with low basal levels of TxnRd1, increases their sensitivity to curcumin alone and to the combination of curcumin and ionizing radiation Homo sapiens