Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
C147A | significantly reduced activity, decrease in FAD content | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
auranofin | - |
Saccharolobus solfataricus | |
azelaic acid | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
NADPH | pH 5.5, 60°C | Saccharolobus solfataricus | |
0.0441 | - |
thioredoxin | pH 5.5, 60°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Saccharolobus solfataricus |
70000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97W27 | enzyme shows additionally NADH oxidase activity | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Saccharolobus solfataricus | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
additional information | enzyme shows additionally NADH oxidase activity | Saccharolobus solfataricus | ? | - |
? | |
thioredoxin + NADP+ | - |
Saccharolobus solfataricus | thioredoxin disulfide + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
NADPH | pH 5.5, 60°C | Saccharolobus solfataricus | |
1.3 | - |
thioredoxin | pH 5.5, 60°C | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | wild-type, 0.7 mol per mol of subunit, mutant C147A, 0.3 mol per mol of subunit | Saccharolobus solfataricus | |
NADP+ | - |
Saccharolobus solfataricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
auranofin | pH 5.5, 60°C | Saccharolobus solfataricus | |
0.054 | - |
azelaic acid | pH 5.5, 60°C | Saccharolobus solfataricus |