Cloned (Comment) | Organism |
---|---|
- |
Medicago truncatula |
expressed in Escherichia coli BL21 (pLysS) cells | Medicago truncatula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
NADPH | - |
Medicago truncatula | |
0.0024 | - |
NADPH | pH 7.5 | Medicago truncatula | |
0.0095 | - |
BAS1 | - |
Medicago truncatula | |
0.011 | - |
NADH | - |
Medicago truncatula | |
0.011 | - |
NADH | pH 7.5 | Medicago truncatula |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Medicago truncatula | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
reducing SDS-PAGE, with His-tag | Medicago truncatula |
52000 | - |
3 * 52000, native MtNTRC seems to be a polymer probably comprising three subunits linked by disulphide bridges, SDS-PAGE | Medicago truncatula |
148000 | - |
gel filtration | Medicago truncatula |
148000 | - |
Superdex 200 gel filtration | Medicago truncatula |
150000 | - |
non-reducing SDS-PAGE | Medicago truncatula |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago truncatula | - |
- |
- |
Medicago truncatula | A6XJ27 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelating Sepharose column chromatography and Superdex 75 column gel filtration | Medicago truncatula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | from dry and germinating seeds | Medicago truncatula | - |
leaf | - |
Medicago truncatula | - |
leaf | NTRC may be involved in the scavenging of peroxides produced in green tissues during the day or the night and in seeds during germination | Medicago truncatula | - |
seed | more abundant in cotyledons from dry and germinating seeds | Medicago truncatula | - |
stem | - |
Medicago truncatula | - |
stem | NTRC may be involved in the scavenging of peroxides produced in green tissues during the day or the night and in seeds during germination | Medicago truncatula | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+ | - |
Medicago truncatula | 2-nitro-5-thiobenzoate + NAD+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | - |
Medicago truncatula | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Medicago truncatula | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
BAS1 + hydrogen peroxide | - |
Medicago truncatula | ? | - |
? | |
thioredoxin + NADP+ | - |
Medicago truncatula | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin disulfide + NADH + H+ | - |
Medicago truncatula | thioredoxin + NAD+ | - |
r | |
thioredoxin disulfide + NADH + H+ | MtNTRC can use either NADPH or NADH as cofactors | Medicago truncatula | thioredoxin + NAD+ | - |
? | |
thioredoxin disulfide + NADPH + H+ | MtNTRC can use either NADPH or NADH as cofactors | Medicago truncatula | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
polymer | in non-reducing SDS-PAGE, NTRC appears as a single band of 150000 Da corresponding to a polymer probably comprising three subunits linked by disulphide bridges | Medicago truncatula |
trimer | 3 * 52000, native MtNTRC seems to be a polymer probably comprising three subunits linked by disulphide bridges, SDS-PAGE | Medicago truncatula |
Synonyms | Comment | Organism |
---|---|---|
MtNTRC | - |
Medicago truncatula |
NADP-thioredoxin reductase C | - |
Medicago truncatula |
NTR | - |
Medicago truncatula |
NTRC | - |
Medicago truncatula |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Medicago truncatula | |
0.62 | - |
5,5'-dithiobis(2-nitrobenzoic acid) | pH 7.5 | Medicago truncatula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Medicago truncatula | |
NADPH | - |
Medicago truncatula |