Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.9 extracted from

  • Alkhalfioui, F.; Renard, M.; Montrichard, F.
    Unique properties of NADP-thioredoxin reductase C in legumes (2006), J. Exp. Bot., 58, 969-978.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Medicago truncatula
expressed in Escherichia coli BL21 (pLysS) cells Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
NADPH
-
Medicago truncatula
0.0024
-
NADPH pH 7.5 Medicago truncatula
0.0095
-
BAS1
-
Medicago truncatula
0.011
-
NADH
-
Medicago truncatula
0.011
-
NADH pH 7.5 Medicago truncatula

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Medicago truncatula 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
reducing SDS-PAGE, with His-tag Medicago truncatula
52000
-
3 * 52000, native MtNTRC seems to be a polymer probably comprising three subunits linked by disulphide bridges, SDS-PAGE Medicago truncatula
148000
-
gel filtration Medicago truncatula
148000
-
Superdex 200 gel filtration Medicago truncatula
150000
-
non-reducing SDS-PAGE Medicago truncatula

Organism

Organism UniProt Comment Textmining
Medicago truncatula
-
-
-
Medicago truncatula A6XJ27
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating Sepharose column chromatography and Superdex 75 column gel filtration Medicago truncatula

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon from dry and germinating seeds Medicago truncatula
-
leaf
-
Medicago truncatula
-
leaf NTRC may be involved in the scavenging of peroxides produced in green tissues during the day or the night and in seeds during germination Medicago truncatula
-
seed more abundant in cotyledons from dry and germinating seeds Medicago truncatula
-
stem
-
Medicago truncatula
-
stem NTRC may be involved in the scavenging of peroxides produced in green tissues during the day or the night and in seeds during germination Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
-
Medicago truncatula 2-nitro-5-thiobenzoate + NAD+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
Medicago truncatula 2-nitro-5-thiobenzoate + NADP+
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
Medicago truncatula 2-nitro-5-thiobenzoate + NADP+
-
?
BAS1 + hydrogen peroxide
-
Medicago truncatula ?
-
?
thioredoxin + NADP+
-
Medicago truncatula thioredoxin disulfide + NADPH
-
?
thioredoxin disulfide + NADH + H+
-
Medicago truncatula thioredoxin + NAD+
-
r
thioredoxin disulfide + NADH + H+ MtNTRC can use either NADPH or NADH as cofactors Medicago truncatula thioredoxin + NAD+
-
?
thioredoxin disulfide + NADPH + H+ MtNTRC can use either NADPH or NADH as cofactors Medicago truncatula thioredoxin + NADP+
-
?

Subunits

Subunits Comment Organism
polymer in non-reducing SDS-PAGE, NTRC appears as a single band of 150000 Da corresponding to a polymer probably comprising three subunits linked by disulphide bridges Medicago truncatula
trimer 3 * 52000, native MtNTRC seems to be a polymer probably comprising three subunits linked by disulphide bridges, SDS-PAGE Medicago truncatula

Synonyms

Synonyms Comment Organism
MtNTRC
-
Medicago truncatula
NADP-thioredoxin reductase C
-
Medicago truncatula
NTR
-
Medicago truncatula
NTRC
-
Medicago truncatula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.62
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
Medicago truncatula
0.62
-
5,5'-dithiobis(2-nitrobenzoic acid) pH 7.5 Medicago truncatula

Cofactor

Cofactor Comment Organism Structure
NADH
-
Medicago truncatula
NADPH
-
Medicago truncatula