Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | under growth conditions | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isoform TrxR1 | Homo sapiens | 5829 | - |
mitochondrion | isoform TrxR2 | Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
selenocysteine | heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine | Homo sapiens | |
selenocysteine | contains selenocysteine as part of the redox active selenosulfide in the active center | Homo sapiens | |
selenocysteine | isoform 1 contains 1 selenium per subunit, the mitochondrial isoform 2 contains 0.5 selenium per subunit | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54800 | - |
2 * 54800, HeLa cell enzyme, MALDI | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
HeLa cells | - |
Oxidation Stability | Organism |
---|---|
the reduced enzyme is very labile to O2, decrease of selenium content and activity after exposure to air, protection by NADPH, NADP+ and tris-(2-carboxyethyl)phosphine | Homo sapiens |
Purification (Comment) | Organism |
---|---|
heparin affinity depends on the selenium content, binding can be induced by reduction of the enzyme with NADPH or tris-(2-carboxyethyl)phosphine | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | conformational change upon reduction wth NADPH or tris-(2-carboxyethyl)phosphine | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | high and low affinity form with respect to heparin | Homo sapiens | - |
HeLa cell | heparin affinity depends on the selenium content | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | i.e. DTNB | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH | coupled assay | Homo sapiens | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Homo sapiens | thioredoxin disulfide + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 54800, HeLa cell enzyme, MALDI | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
TrxR | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |