Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes from plasmids | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C136S | site-directed mutagenesis, reduced activity | Escherichia coli |
C139S | site-directed mutagenesis, reduced activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00088 | - |
thioredoxin | mutant C136S | Escherichia coli | |
0.00125 | - |
thioredoxin | wild-type | Escherichia coli | |
0.0017 | - |
thioredoxin | mutant C139S | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
enzyme with active site mutation | - |
Purification (Comment) | Organism |
---|---|
wild-type from overexpressing cell line, mutants | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+ | catalytic mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thioredoxin + NADP+ | coupled assay with DTNB | Escherichia coli | thioredoxin disulfide + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TR | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.38 | - |
thioredoxin | mutant C136S | Escherichia coli | |
13.2 | - |
thioredoxin | mutant C139S | Escherichia coli | |
22 | - |
thioredoxin | wild-type | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |