Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.7 extracted from

  • Yadav, S.S.; Srikanth, E.; Singh, N.; Rathaur, S.
    Identification of GR and TrxR systems in Setaria cervi: Purification and characterization of glutathione reductase (2013), Parasitol. Int., 62, 193-198.
    View publication on PubMed
Search for more literature for 1.8.1.7

Inhibitors

Inhibitors Comment Organism Structure
2,4-dihydroxybenzylamine specific inhibitor, complete inhibition at 0.001 mM Setaria cervi
diethyldicarbonate
-
 Setaria cervi
N-Acetylimidazole
-
 Setaria cervi
N-ethylmaleimide
-
 Setaria cervi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 NADPH in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi
0.59
-
 glutathione disulfide in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000, SDS-PAGE Setaria cervi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH + H+ Setaria cervi
-
 glutathione + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Setaria cervi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2',5'-ADP agarose affinity chromatography and DEAE Sephadex gel filtration Setaria cervi

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Setaria cervi
-
larva
-
 Setaria cervi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.16
-
 homogenate, in 50 mM potassium phosphate buffer pH 7.0, at 25°C Setaria cervi
643
-
 89.8fold purified enzyme, in 50 mM potassium phosphate buffer pH 7.0, at 25°C Setaria cervi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
 Setaria cervi glutathione + NADP+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 50000, SDS-PAGE Setaria cervi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Setaria cervi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Setaria cervi

Cofactor

Cofactor Comment Organism Structure
flavin
-
 Setaria cervi
NADPH
-
 Setaria cervi

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.27
-
 in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi diethyldicarbonate
0.5
-
 in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi N-ethylmaleimide
0.71
-
 in 50 mM potassium phosphate buffer pH 7.0, at 37°C Setaria cervi N-Acetylimidazole