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Literature summary for 1.8.1.7 extracted from

  • Arora, K.; Ahmad, R.; Srivastava, A.
    Purification and characterization of glutathione reductase (E.C. 1.8.1.7) from bovine filarial worms Setaria cervi (2013), J. Parasit. Dis., 37, 94-104.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
glutathione product inhibition Setaria cervi
K+ inhibitory in 0.1-1.0 M concentration range Setaria cervi
Na+ inhibitory in 0.1-1.0 M concentration range Setaria cervi
NADP+ product inhibition Setaria cervi
NH4+ inhibitory in 0.1-1.0 M concentration range Setaria cervi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
NADPH crude enzyme, at pH 7.0 and 22°C Setaria cervi
0.021
-
NADPH purified enzyme, at pH 7.0 and 22°C Setaria cervi
0.03
-
NADP+ purified enzyme at pH 7.0 and 22°C Setaria cervi
0.0465
-
glutathione disulfide crude enzyme, at pH 7.0 and 22°C Setaria cervi
0.048
-
NADP+ crude enzyme, at pH 7.0 and 22°C Setaria cervi
0.05
-
glutathione disulfide purified enzyme, at pH 7.0 and 22°C Setaria cervi
5.9
-
glutathione purified enzyme, at pH 7.0 and 22°C Setaria cervi
6.8
-
glutathione crude enzyme, at pH 7.0 and 22°C Setaria cervi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
2 * 55000, SDS-PAGE Setaria cervi
110000
-
gel filtration Setaria cervi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutathione disulfide + NADPH + H+ Setaria cervi
-
glutathione + NADP+
-
ir

Organism

Organism UniProt Comment Textmining
Setaria cervi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, ultrafiltration, CM-Sepharose column chromatography, DEAE-Sepharose column chromatography, GSH–Sepharose affinity column chromatography, and ADP-Sepharose affinity chromatography Setaria cervi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0362
-
purified enzyme, at pH 7.0 and 22°C Setaria cervi

Storage Stability

Storage Stability Organism
0-4°C, 1 month, no appreciable effect of freezing and thawing on enzyme activity Setaria cervi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
Setaria cervi glutathione + NADP+
-
ir

Subunits

Subunits Comment Organism
homodimer 2 * 55000, SDS-PAGE Setaria cervi

Synonyms

Synonyms Comment Organism
glutathione: NADP+ oxidoreductase
-
Setaria cervi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
4 there is no appreciable effect of freezing and thawing on enzyme activity for approximately 1 month Setaria cervi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 broad pH optimum centered around pH 7.0 Setaria cervi

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Setaria cervi