Literature summary for 1.8.1.7 extracted from

Arias, D.G.; Marquez, V.E.; Beccaria, A.J.; Guerrero, S.A.; Iglesias, A.A.
Purification and characterization of a glutathione reductase from Phaeodactylum tricornutum (2010), Protist, 161, 91-101.

Search for more literature for 1.8.1.7

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	GRase-1 is moderately sensitive to inhibition by Co2+	Phaeodactylum tricornutum
Cu2+	GRase-1 is very sensitive to inhibition by Cu2+	Phaeodactylum tricornutum
glutathione	GSH functions as an inhibitor at relatively high concentrations (41 mM), and also only in the lower substrate concentration range	Phaeodactylum tricornutum
NADP+	competitive product inhibition regarding NADPH and non-competitive product inhibition regarding glutathione disulfide, however, NADP+ (up to 1m M) is not inhibitor when assays are performed at 1 mM glutathione disulfide and 300 mM NADPH	Phaeodactylum tricornutum
Ni2+	-	Phaeodactylum tricornutum
Zn2+	GRase-1 is very sensitive to inhibition by Zn2+	Phaeodactylum tricornutum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.014	-	NADPH	at 30°C and pH 7.5	Phaeodactylum tricornutum
0.06	-	glutathione disulfide	at 30°C and pH 7.5	Phaeodactylum tricornutum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	composed of two major proteins of 58000 and 40000 Da, SDS-PAGE	Phaeodactylum tricornutum
53470	-	subunit, calculated from amino acid sequence	Phaeodactylum tricornutum
58000	-	composed of two major proteins of 58000 and 40000 Da, SDS-PAGE	Phaeodactylum tricornutum
59000	-	2 * 59000, native PAGE	Phaeodactylum tricornutum
118000	-	native molecular mass, native PAGE	Phaeodactylum tricornutum

Organism

Organism	UniProt	Comment	Textmining
Phaeodactylum tricornutum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Blue-A Sepharose column chromatography, and 2',5'-ADP-Sepharose column chromatography	Phaeodactylum tricornutum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.46	-	enzyme from soluble extract, at 30°C and pH 7.5	Phaeodactylum tricornutum
73.3	-	after 161fold purification, at 30°C and pH 7.5	Phaeodactylum tricornutum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glutathione disulfide + NADPH + H+	-	Phaeodactylum tricornutum	glutathione + NADP+	using levels up to 300 mM NADP+ and 1 mM glutathione, the enzyme does not catalyse the reverse reaction, indicating that its physiological function is the reduction of GSSG or GSNO but not the opposite	ir
additional information	the enzyme is not effective to reduce cystine, lipoamide, nor trypanothione disulfide	Phaeodactylum tricornutum	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 59000, native PAGE	Phaeodactylum tricornutum

Synonyms

Synonyms	Comment	Organism
glutathione reductase	-	Phaeodactylum tricornutum
glutathione:NADP+ oxidoreductase	-	Phaeodactylum tricornutum
GRase-1	-	Phaeodactylum tricornutum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Phaeodactylum tricornutum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	35	the abrupt decrease in activity observed at temperatures higher than 35°C, can be associated with loss of enzyme stability beyond 30°C	Phaeodactylum tricornutum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Phaeodactylum tricornutum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	10	in 100 mM Tris-HCl buffer	Phaeodactylum tricornutum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6.5	10	the enzyme remains stable in 100 mM Tris-HCl buffer between pH 6.5 and 10.0, the activity remains practically unchanged between pH 6.5-7.5 and decreases significantly only above pH 8.5	Phaeodactylum tricornutum

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	utilizes NADPH but not NADH as electron donor	Phaeodactylum tricornutum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.052	-	NADP+	competitive product inhibition regarding NADPH, at 30°C and pH 7.5	Phaeodactylum tricornutum
1	-	NADP+	non-competitive product inhibition regarding glutathione disulfide, at 30°C and pH 7.5	Phaeodactylum tricornutum
5	-	glutathione	competitive product inhibition regarding glutathione disulfide, at 30°C and pH 7.5	Phaeodactylum tricornutum
10	-	glutathione	non-competitive product inhibition regarding NADPH, at 30°C and pH 7.5	Phaeodactylum tricornutum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Phaeodactylum tricornutum	calculated from amino acid sequence	-	5

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0034	-	at 30°C and pH 7.5	Phaeodactylum tricornutum	Cu2+
0.0042	-	at 30°C and pH 7.5	Phaeodactylum tricornutum	Zn2+
0.036	-	at 30°C and pH 7.5	Phaeodactylum tricornutum	Co2+
0.534	-	at 30°C and pH 7.5	Phaeodactylum tricornutum	Ni2+

General Information

General Information	Comment	Organism
physiological function	the enzyme's physiological function is the reduction of glutathione disulfide or GSNO but not the opposite	Phaeodactylum tricornutum

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Release 2023.1 (January 2023)