Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | Ipomoea batatas |
General Stability | Organism |
---|---|
active in the presence of imidazole up to 0.8 M, the native enzyme is resistant to digestion by trypsin or chymotrypsin | Ipomoea batatas |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | in the presence of 1 mM Cu2+, approximately 5% of residual activity is detected | Ipomoea batatas | |
additional information | not inhibited by Ca2+, addition of imidazole up to 0.8 M has no significant effect on the activity | Ipomoea batatas | |
Zn2+ | in the presence of 1 mM Zn2+, approximately 55% of residual activity is detected | Ipomoea batatas |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
NADPH | in 100 mM potassium phosphate buffer (pH 8.0), at 25°C | Ipomoea batatas | |
0.114 | - |
glutathione disulfide | in 100 mM potassium phosphate buffer (pH 8.0), at 25°C | Ipomoea batatas |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54600 | - |
quadrupol time of flight electrospray ionization mass spectrometry | Ipomoea batatas |
54800 | - |
1 * 54800, active monomeric form, SDS-PAGE | Ipomoea batatas |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ipomoea batatas | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Ipomoea batatas |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Ipomoea batatas | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | - |
Ipomoea batatas | glutathione + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54800, active monomeric form, SDS-PAGE | Ipomoea batatas |
Synonyms | Comment | Organism |
---|---|---|
EC 1.6.4.2 | formerly | Ipomoea batatas |
glutathione reductase | - |
Ipomoea batatas |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
the protein's half-life of deactivation at 70°C is 3.3 min | Ipomoea batatas |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50.14 | - |
glutathione disulfide | in 100 mM potassium phosphate buffer (pH 8.0), at 25°C | Ipomoea batatas | |
53.72 | - |
NADPH | in 100 mM potassium phosphate buffer (pH 8.0), at 25°C | Ipomoea batatas |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Ipomoea batatas |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
glutathione reductase has little or no activity at pH 4.0 or lower | Ipomoea batatas |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Ipomoea batatas | |
NADPH | - |
Ipomoea batatas |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
439.8 | - |
glutathione disulfide | in 100 mM potassium phosphate buffer (pH 8.0), at 25°C | Ipomoea batatas | |
959.3 | - |
NADPH | in 100 mM potassium phosphate buffer (pH 8.0), at 25°C | Ipomoea batatas |