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Literature summary for 1.8.1.7 extracted from

  • Chen, C.J.; Huang, C.Y.; Huang, J.K.; Lin, C.Y.; Lin, C.T.
    Cloning, expression, and purification of a functional glutathione reductase from sweet potato (Ipomoea batatas [L.] Lam): Kinetic studies and characterization (2009), J. Agric. Food Chem., 57, 4403-4408.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Ipomoea batatas

General Stability

General Stability Organism
active in the presence of imidazole up to 0.8 M, the native enzyme is resistant to digestion by trypsin or chymotrypsin Ipomoea batatas

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ in the presence of 1 mM Cu2+, approximately 5% of residual activity is detected Ipomoea batatas
additional information not inhibited by Ca2+, addition of imidazole up to 0.8 M has no significant effect on the activity Ipomoea batatas
Zn2+ in the presence of 1 mM Zn2+, approximately 55% of residual activity is detected Ipomoea batatas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
NADPH in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas
0.114
-
glutathione disulfide in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54600
-
quadrupol time of flight electrospray ionization mass spectrometry Ipomoea batatas
54800
-
1 * 54800, active monomeric form, SDS-PAGE Ipomoea batatas

Organism

Organism UniProt Comment Textmining
Ipomoea batatas
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Ipomoea batatas

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Ipomoea batatas
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione disulfide + NADPH + H+
-
Ipomoea batatas glutathione + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 54800, active monomeric form, SDS-PAGE Ipomoea batatas

Synonyms

Synonyms Comment Organism
EC 1.6.4.2 formerly Ipomoea batatas
glutathione reductase
-
Ipomoea batatas

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
the protein's half-life of deactivation at 70°C is 3.3 min Ipomoea batatas

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50.14
-
glutathione disulfide in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas
53.72
-
NADPH in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
Ipomoea batatas

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
glutathione reductase has little or no activity at pH 4.0 or lower Ipomoea batatas

Cofactor

Cofactor Comment Organism Structure
FAD
-
Ipomoea batatas
NADPH
-
Ipomoea batatas

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
439.8
-
glutathione disulfide in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas
959.3
-
NADPH in 100 mM potassium phosphate buffer (pH 8.0), at 25°C Ipomoea batatas