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Literature summary for 1.8.1.7 extracted from
- Purification and kinetic properties of glutathione reductase from bovine liver (2007), Mol. Cell. Biochem., 303, 45-51.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GSH | With regard to GSSG as variable substrate at fixed NADPH concentration (0.1 mM), GSH is a non-competitive inhibitor. With regard to NADPH as variable substrate at fixed GSSG concentration, GSH is a non-competitive inhibitor | Bos taurus |  |
| NADP+ | With regard to GSSG as variable substrate at fixed NADPH concentration, NADP+ is an uncompetitive inhibitor. With regard to NADPH as variable substrate at fixed GSSG concentration, NADP+ is a competitive inhibitor | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
NADPH | pH 7.4, 37°C | Bos taurus | |
| 0.154 | - |
glutathione disulfide | pH 7.4, 37°C | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | the enzyme follows the Ping Pong mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 185.5 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH + H+ | - |
Bos taurus | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.043 | - |
NADP+ | pH 7.4, 37°C, NADPH as variable substrate at fixed GSSG concentration | Bos taurus | |
| 0.219 | - |
NADP+ | pH 7.4, 37°C, GSSG as variable substrate at fixed NADPH concentration (0.1 mM) | Bos taurus | |
| 7.985 | - |
GSH | pH 7.4, 37°C, GSSG as variable substrate at fixed NADPH concentration (0.1 mM) | Bos taurus | |
| 8.506 | - |
GSH | pH 7.4, 37°C, NADPH as variable substrate at fixed GSSG concentration | Bos taurus | |
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Release 2023.1 (January 2023)
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