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Literature summary for 1.8.1.7 extracted from
- Isolation and characterization of glutathione reductase from Physarum polycephalum and stage-specific expression of the enzyme in life-cycle stages with different oxidation-reduction levels (2003), J. Eukaryot. Microbiol., 50, 317-323.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of a genetic library, DNA and amino acid sequence determination and analysis of the enzyme encoding gene, expression in Escherichia coli | Physarum polycephalum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0286 | - |
NADPH | - |
Physarum polycephalum |  |
| 0.04 | - |
glutathione disulfide | - |
Physarum polycephalum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49300 | - |
2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry | Physarum polycephalum |
| 50000 | - |
2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry | Physarum polycephalum |
| 99200 | - |
velocity sedimentation analysis | Physarum polycephalum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH | Physarum polycephalum | - |
glutathione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Physarum polycephalum | Q9NL96 | strain ATCC 24466 and 24467 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from microplasmodia, 968fold in a multistep procedure | Physarum polycephalum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| microplasmodium | - |
Physarum polycephalum | - |
| additional information | stage-specific expression of the enzyme in life-cycle stages with different oxidation-reduction levels, glutathione levels, overview | Physarum polycephalum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 484 | - |
purified native enzyme | Physarum polycephalum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADPH | - |
Physarum polycephalum | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, SDS-PAGE, 2 * 49300, MALDI-TOF mass spectrometry | Physarum polycephalum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutathione reductase | - |
Physarum polycephalum |
| GR | - |
Physarum polycephalum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Physarum polycephalum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Physarum polycephalum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Physarum polycephalum | |
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Release 2023.1 (January 2023)
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