Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | redox active disulfide at the catalytic site | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
mutant enzyme glutathione reductase-trypanothine reductase, expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
substrate-binding and structure analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A34E/R37W | glutathione reductase mutant, activity switches to trypanothione reductase, termed GRTR, 700fold more activity with trypanothione than with glutathione | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
GSSG | - |
Homo sapiens | |
0.5 | - |
trypanothione | recombinant glutathione-trypanothione reductase-like enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00390 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | member of pyridine-nucleotide disulfide oxidoreductase family of flavoenzymes | Homo sapiens | |
2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | recombinant glutathione-trypanothione reductase-like enzyme, substrate binding, three dimensional structure, complex formation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GSSG + NADPH | - |
Homo sapiens | glutathione + NADP+ | - |
? | |
trypanothione + NADPH | recombinant glutathione-trypanothione reductase-like enzyme | Homo sapiens | reduced trypanothione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GR | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.8 | - |
trypanothione | recombinant glutathione-trypanothione reductase-like enzyme | Homo sapiens | |
210 | - |
GSSG | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant glutathione-trypanothione reductase-like enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |