Any feedback?
Literature summary for 1.8.1.7 extracted from
- Horse-liver glutathione reductase: purification and characterization (1993), Int. J. Biochem., 25, 61-68.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Equus caballus |  |
| KI | - |
Equus caballus | |
| KNO3 | - |
Equus caballus | |
| Na2S2O3 | - |
Equus caballus | |
| NaCl | - |
Equus caballus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | different isozymes, temperature-dependent, homo-glutathione | Equus caballus | |
| 0.009 | - |
NADPH | - |
Equus caballus | |
| 0.059 | - |
GSSG | - |
Equus caballus | |
| 0.56 | - |
ferricyanide | - |
Equus caballus | |
| 0.68 | - |
NADH | - |
Equus caballus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KBr | below 0.1 M | Equus caballus | |
| KH2PO4 | below 0.1 M | Equus caballus | |
| NaF | below 0.1 M | Equus caballus | |
| NH4Cl | below 0.1 M | Equus caballus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 107000 | - |
native PAGE | Equus caballus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Equus caballus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Equus caballus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 248 | - |
- |
Equus caballus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferricyanide + NADPH + H+ | - |
Equus caballus | ferrocyanide + NADP+ | - |
r | |
| glutathione disulfide + NADH + H+ | - |
Equus caballus | glutathione + NAD+ | - |
? | |
| GSSG + NADPH | ferricyanide and 2,6-dichlorophenolindophenol as electron acceptors | Equus caballus | glutathione + NADP+ | - |
r | |
| GSSG + NADPH | mixed disulfide between coenzyme A and glutathione | Equus caballus | glutathione + NADP+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
NADH + glutathione | Equus caballus |
| 7 | - |
NADPH + GSSG | Equus caballus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| deamino-NADPH | 69% of the activity with NADPH | Equus caballus | |
| ferricyanide | - |
Equus caballus | |
| flavin | flavoprotein | Equus caballus | |
| additional information | total lack of activity with 3-acetylpyridine NADH | Equus caballus | |
| NADH | 8.5% of the activity with NADPH | Equus caballus | |
| NADPH | - |
Equus caballus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
