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Literature summary for 1.8.1.7 extracted from
- Glutathione reductase in the sea urchin egg. I. Purification and general properties (1974), Biochim. Biophys. Acta, 350, 141-150.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Iodide | - |
Hemicentrotus pulcherrimus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00078 | - |
FAD | - |
Hemicentrotus pulcherrimus | |
| 0.005 | - |
NADPH | - |
Hemicentrotus pulcherrimus | |
| 0.101 | - |
GSSG | - |
Hemicentrotus pulcherrimus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | optimum concentration of potassium phosphate: 0.1 M | Hemicentrotus pulcherrimus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Hemicentrotus pulcherrimus |
| 102000 | - |
gel filtration | Hemicentrotus pulcherrimus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hemicentrotus pulcherrimus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hemicentrotus pulcherrimus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ | mechanism | Hemicentrotus pulcherrimus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Hemicentrotus pulcherrimus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 320 | - |
purified enzyme | Hemicentrotus pulcherrimus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -28°C, 50 mM phosphate buffer, a few months | Hemicentrotus pulcherrimus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GSSG + NADPH | - |
Hemicentrotus pulcherrimus | glutathione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52000, SDS-PAGE | Hemicentrotus pulcherrimus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Hemicentrotus pulcherrimus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.3 | - |
Hemicentrotus pulcherrimus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | FAD enzyme | Hemicentrotus pulcherrimus | |
| additional information | no activity with FMN | Hemicentrotus pulcherrimus | |
| NADH | less than 1% activity of that with NADPH | Hemicentrotus pulcherrimus | |
| NADPH | - |
Hemicentrotus pulcherrimus | |
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Release 2023.1 (January 2023)
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