Any feedback?
Literature summary for 1.8.1.7 extracted from
- Purification and properties of the glutathione reductase of Chromatium vinosum (1975), J. Bacteriol., 123, 203-211.
General Stability
| General Stability | Organism |
|---|---|
| repeated freezing and thawing, stable | Allochromatium vinosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Allochromatium vinosum |  |
| AgNO3 | - |
Allochromatium vinosum | |
| AMP | - |
Allochromatium vinosum | |
| ATP | - |
Allochromatium vinosum | |
| BaCl2 | - |
Allochromatium vinosum | |
| Ca2+ | - |
Allochromatium vinosum | |
| Cl- | - |
Allochromatium vinosum | |
| Cu2+ | - |
Allochromatium vinosum | |
| Fe(NO3)2 | - |
Allochromatium vinosum | |
| glutathione | product inhibition | Allochromatium vinosum | |
| KI | - |
Allochromatium vinosum | |
| Mg2+ | - |
Allochromatium vinosum | |
| Mn2+ | - |
Allochromatium vinosum | |
| N-ethylmaleimide | - |
Allochromatium vinosum | |
| p-hydroxymercuribenzoate | - |
Allochromatium vinosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00052 | - |
FAD | - |
Allochromatium vinosum | |
| 0.6 | - |
NADH | - |
Allochromatium vinosum | |
| 7 | - |
GSSG | - |
Allochromatium vinosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | activates | Allochromatium vinosum | |
| Na+ | activates | Allochromatium vinosum | |
| NH4+ | activates | Allochromatium vinosum | |
| phosphate | activates | Allochromatium vinosum | |
| SO42- | stimulates | Allochromatium vinosum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Allochromatium vinosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Allochromatium vinosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.4 | - |
- |
Allochromatium vinosum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2 years | Allochromatium vinosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione disulfide + NADH + H+ | - |
Allochromatium vinosum | glutathione + NAD+ | - |
? | |
| GSSG + NADPH | no activity | Allochromatium vinosum | glutathione + NADP+ | - |
? | |
| additional information | oxidized lipoamide and oxidized lipoic acid | Allochromatium vinosum | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
5 min, 60% loss of activity | Allochromatium vinosum |
| 65 | - |
5 min, complete loss of activity | Allochromatium vinosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
NADH + GSSG | Allochromatium vinosum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | FAD enzyme | Allochromatium vinosum | |
| NADH | specific for NADH | Allochromatium vinosum | |
| NADPH | - |
Allochromatium vinosum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
