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Literature summary for 1.8.1.4 extracted from

  • Nishimoto, E.; Aso, Y.; Koga, T.; Yamashita, S.
    Thermal unfolding process of dihydrolipoamide dehydrogenase studied by fluorescence spectroscopy (2006), J. Biochem., 140, 349-357.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + NAD+
-
Geobacillus stearothermophilus lipoamide + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
Geobacillus stearothermophilus
LipDH
-
Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
the enzyme unfolds irreversibly at heat treatment higher than 65°C Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Geobacillus stearothermophilus
NAD+
-
Geobacillus stearothermophilus