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Literature summary for 1.8.1.4 extracted from

  • Kim, H.
    Activity of human dihydrolipoamide dehydrogenase is reduced by mutation at threonine-44 of FAD-binding region to valine (2002), J. Biochem. Mol. Biol., 35, 437-441.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant T44V in Escherichia coli strain XL1-Blue Homo sapiens

Protein Variants

Protein Variants Comment Organism
T44V site-directed mutagenesis, mutation of Thr44 of the FAD-binding region to Val, corresponding to the prokaryotic sequence, results in 2.2fold reduced activity with a slightly different microenvironment at the FAD-binding site Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50216
-
2 * 50216, amino acid sequence calculation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant T44V from Escherichia coli strain XL1-Blue Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
280
-
purified recombinant mutant T44V enzyme Homo sapiens

Subunits

Subunits Comment Organism
dimer 2 * 50216, amino acid sequence calculation Homo sapiens

Synonyms

Synonyms Comment Organism
dihydrolipoamide dehydrogenase
-
Homo sapiens
dihydrolipoamide:NAD+ oxidoreductase
-
Homo sapiens
E3
-
Homo sapiens
More enzyme is a member of the pyridine nucleotide-disulfide oxidoreductase family of enzymes, enzyme is the E3 component of three different 2-ketoacid dehydrogenase multienzyme complexes, i.e. the pyruvate, 2-ketoglutarate, and branched chain 2-keto acid dehydrogenase complexes Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD 1 FAD as prosthetic group per enzyme subunit Homo sapiens