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Literature summary for 1.8.1.4 extracted from

  • Xia, L.; Björnstedt, M.; Nordman, T.; Eriksson, L.C.; Olsson, J.M.
    Reduction of ubiquinone by lipoamide dehydrogenase. An antioxidant regenerating pathway (2001), Eur. J. Biochem., 268, 1486-1490.
    View publication on PubMed
Search for more literature for 1.8.1.4

Inhibitors

Inhibitors Comment Organism Structure
NAD(P)+ product inhibition Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ highly, specifically stimulating for ubiquinone reduction, optimal at 0.5 mM Sus scrofa
Zn2+ highly, specifically stimulating for ubiquinone reduction, optimal at 0.5 mM, Zn2+ slightly elevates the pH-optimum of the enzyme Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 2 * 55000, about, SDS-PAGE Sus scrofa
115000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ubiquinone + NAD(P)H Sus scrofa
-
 ubiquinol + NAD(P)+
-
 ir

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation purified enzyme Sus scrofa
-
heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 purified enzyme in absence of Zn2+ Sus scrofa
0.05
-
 purified enzyme in presence of Zn2+ Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquinone + NAD(P)H
-
 Sus scrofa ubiquinol + NAD(P)+
-
 ir

Subunits

Subunits Comment Organism
dimer 2 * 55000, about, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
lipoamide dehydrogenase
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
72
-
 ubiquinone pH 7.5, 37°C, in absence of Zn2+ Sus scrofa
300
-
 ubiquinone pH 7.5, 37°C, in presence of Zn2+ Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 optimum in absence of Zn2+ Sus scrofa
6
-
 optimum in presence of Zn2+ Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Sus scrofa
NAD(P)H NADPH and NADH are equally effective as cofactors Sus scrofa