Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
2 * 51000, SDS-PAGE | Malbranchea pulchella |
102000 | - |
gel filtration | Malbranchea pulchella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malbranchea pulchella | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Malbranchea pulchella |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Malbranchea pulchella | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Malbranchea pulchella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + NAD+ | - |
Malbranchea pulchella | lipoamide + NADH | - |
? | |
additional information | diaphorase activity | Malbranchea pulchella | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 51000, SDS-PAGE | Malbranchea pulchella |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
170 min, 50% loss of activity | Malbranchea pulchella |
70 | - |
50 min, 50% loss of activity | Malbranchea pulchella |
75 | - |
50% loss of activity after 15 min, 50% loss of activity after 20 min in presence of NAD+, 50% loss of activity after less than 1 min in presence of dihydrolipoamide | Malbranchea pulchella |
80 | - |
6 min, 50% loss of activity | Malbranchea pulchella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Malbranchea pulchella |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5.0: about 10% of maximal activity, pH 7.0-7.5: 60-70% of maximal activity, pH 8.0-8.5: 90% of maximal activity, pH 10.0: 10% of maximal activity in carbonate buffer | Malbranchea pulchella |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains one noncovalently bound FAD per subunit | Malbranchea pulchella | |
NAD+ | - |
Malbranchea pulchella |