Literature summary for 1.8.1.2 extracted from

Cordente, A.G.; Heinrich, A.; Pretorius, I.S.; Swiegers, J.H.
Isolation of sulfite reductase variants of a commercial wine yeast with significantly reduced hydrogen sulfide production (2009), FEMS Yeast Res., 9, 446-459.

Search for more literature for 1.8.1.2

Application

Application	Comment	Organism
brewing	mutations in the sulfite reductase genes, MET5 and MET10, are responsible for low H2S phenotypes, yeast strains with reduced H2S production offer promising solutions to H2S-related taints generated during wine production	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
the MET5 and Met10 coding sequences are cloned into the vector pGEM-T-easy and subsequently into the centromeric Saccharomyces cerevisiae expression plasmid pRS416	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
A979T	Met5p mutant	Saccharomyces cerevisiae
E1356K	Met5p mutant	Saccharomyces cerevisiae
E929K	Met10p mutant	Saccharomyces cerevisiae
G1115D	Met5p mutant	Saccharomyces cerevisiae
L606F	Met10p mutant	Saccharomyces cerevisiae
T990I	Met10p mutant	Saccharomyces cerevisiae
T997I	Met10p mutant	Saccharomyces cerevisiae
W59X	Met10p mutant	Saccharomyces cerevisiae
W841X	Met10p mutant	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
hydrogen sulfide + NADP+ + H2O	Saccharomyces cerevisiae	-	sulfite + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	allele 1, subunit beta, MET5, subunit alpha, MET10	-
Saccharomyces cerevisiae	-	allele 2, subunit beta, MET5, subunit alpha, MET10	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hydrogen sulfide + NADP+ + H2O	-	Saccharomyces cerevisiae	sulfite + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
heterotetramer	two alpha- and two beta-subunits, encoded by the MET10 and MET5 gene, respectively	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
sulfite reductase	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Saccharomyces cerevisiae
FMN	-	Saccharomyces cerevisiae
iron-sulfur centre	-	Saccharomyces cerevisiae
NADPH	-	Saccharomyces cerevisiae
siroheme	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)