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Literature summary for 1.8.1.2 extracted from
- TPNH-Sulfite reductase (Escherichia coli) (1971), Methods Enzymol., 17B, 539-545.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| arsenite | - |
Escherichia coli |  |
| CN- | - |
Escherichia coli | |
| CO | - |
Escherichia coli | |
| NADP+ | NADPH-dependent activities | Escherichia coli | |
| p-Mercuriphenylsulfonate | NADPH-dependent reactions | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0043 | 0.0074 | sulfite | - |
Escherichia coli | |
| 0.018 | - |
NADPH | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.73 | 2.87 | - |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 0.05 M potassium phosphate buffer pH 7.7, 10 mM EDTA, at least 6 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + NADPH | - |
Escherichia coli | ammonia + NADP+ | - |
? | |
| additional information | reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 | Escherichia coli | ? | - |
? | |
| nitrite + NADPH | - |
Escherichia coli | ammonia + NADP+ | - |
? | |
| sulfite + NADPH | - |
Escherichia coli | sulfide + NADP+ + H2O | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | 40 | sulfite | cosubstrate NADPH | Escherichia coli | |
| 43.3 | 51.7 | nitrite | cosubstrate NADPH | Escherichia coli | |
| 98.3 | 217 | hydroxylamine | cosubstrate NADPH | Escherichia coli | |
| 150 | - |
FAD | cosubstrate NADPH | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
electron donor NADPH | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | 0.009 | cyanide | - |
Escherichia coli | |
| 0.08 | 0.12 | arsenite | - |
Escherichia coli | |
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