Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.2 extracted from

  • Ostrowski, J.; Barber, M.J.; Rueger, D.C.; Miller, B.E.; Siegel, L.M.; Kredich, N.M.
    Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrome P-450 reductase (1989), J. Biol. Chem., 264, 15796-15808.
    View publication on PubMed
Search for more literature for 1.8.1.2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
488000
-
 sedimentation under nondenaturing conditions Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

Reaction Comment Organism Reaction ID
hydrogen sulfide + 3 NADP+ + 3 H2O = sulfite + 3 NADPH + 3 H+ electron flow sequence: NADPH-FAD-FMN Salmonella enterica subsp. enterica serovar Typhimurium
a
hydrogen sulfide + 3 NADP+ + 3 H2O = sulfite + 3 NADPH + 3 H+ electron flow sequence: NADPH-FAD-FMN Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
81
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate Escherichia coli ?
-
 ?
additional information reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit Escherichia coli ?
-
 ?
additional information reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 Escherichia coli ?
-
 ?
nitrite + NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium ammonia + NADP+
-
 ?
nitrite + NADPH
-
 Escherichia coli ammonia + NADP+
-
 ?
sulfite + NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium sulfide + NADP+ + H2O
-
 ?
sulfite + NADPH
-
 Escherichia coli sulfide + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dodecamer alpha8,beta4, 8 * 58000-60000 + 4 * 54000-57000, sedimentation under denaturing conditions Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information values for reactions catalyzed by holoenzyme, hemoprotein subunit alone, and flavoprotein subunit alone with various electron acceptors Salmonella enterica subsp. enterica serovar Typhimurium
additional information
-
 additional information values for reactions catalyzed by holoenzyme, hemoprotein subunit alone, and flavoprotein subunit alone with various electron acceptors Escherichia coli

Cofactor

Cofactor Comment Organism Structure
flavin 4 FAD and 4 FMN per mol of enzyme Salmonella enterica subsp. enterica serovar Typhimurium
flavin 4 FAD and 4 FMN per mol of enzyme Escherichia coli