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Literature summary for 1.8.1.2 extracted from

  • Hatefi, Y.; Stiggall, D.L.
    Metal-containing flavoprotein dehydrogenases (1976), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 13, 175-297.
No PubMed abstract available
Search for more literature for 1.8.1.2

Inhibitors

Inhibitors Comment Organism Structure
arsenite
-
 Escherichia coli
arsenite
-
 Saccharomyces cerevisiae
CN-
-
 Escherichia coli
CN-
-
 Neurospora crassa
CN-
-
 Saccharomyces cerevisiae
CN-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
CO
-
 Escherichia coli
CO
-
 Neurospora crassa
CO
-
 Saccharomyces cerevisiae
CO
-
 Salmonella enterica subsp. enterica serovar Typhimurium
p-Mercuriphenylsulfonate NADPH-dependent reactions Escherichia coli
Sulfide reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen Escherichia coli
Sulfide reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen Saccharomyces cerevisiae
Sulfide reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0043 0.0074 sulfite
-
 Escherichia coli
0.005
-
 NADPH
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Neurospora crassa
-
-
-
Saccharomyces cerevisiae
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + NADPH
-
 Escherichia coli ammonia + NADP+
-
 ?
additional information transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate Escherichia coli ?
-
 ?
additional information transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate Saccharomyces cerevisiae ?
-
 ?
additional information reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit Escherichia coli ?
-
 ?
additional information reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit Saccharomyces cerevisiae ?
-
 ?
additional information reactions of FMN depleted enzyme Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information reactions of FMN depleted enzyme Escherichia coli ?
-
 ?
additional information reactions of FMN depleted enzyme Saccharomyces cerevisiae ?
-
 ?
additional information reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
additional information reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 Escherichia coli ?
-
 ?
additional information reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 Saccharomyces cerevisiae ?
-
 ?
nitrite + NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium ammonia + NADP+
-
 ?
nitrite + NADPH
-
 Escherichia coli ammonia + NADP+
-
 ?
nitrite + NADPH
-
 Saccharomyces cerevisiae ammonia + NADP+
-
 ?
nitrite + NADPH
-
 Neurospora crassa ammonia + NADP+
-
 ?
sulfite + NADPH reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone Salmonella enterica subsp. enterica serovar Typhimurium sulfide + NADP+ + H2O
-
 ?
sulfite + NADPH reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone Escherichia coli sulfide + NADP+ + H2O
-
 ?
sulfite + NADPH reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone Saccharomyces cerevisiae sulfide + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dodecamer alpha8,beta4, 8 * 58000-60000 + 4 * 54000-57000, sedimentation under denaturing conditions Escherichia coli
More dissociation of enzyme by 5 m urea into flavoprotein octamer and hemoprotein monomers Escherichia coli