Any feedback?
Literature summary for 1.8.1.12 extracted from
- Trypanothione reductase from Leishmania donovani. Purification, characterization and inhibition by trivalent antimonials (1995), Eur. J. Biochem., 230, 460-468.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | activity is highly dependent on ionic strength, optimum activity at 40 mM NaCl | Leishmania donovani | |
| additional information | activity is dependent on a redox-active cysteine at the active centre | Leishmania donovani |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | target for rational approach to design new anti-leishmanial chemotherapeutic agents | Leishmania donovani |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into expression vector and expressed in wild-type cells | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antimony chloride | assay containing 10% vol. methanol | Leishmania donovani |  |
| melarsen-trypanothione disulfide adduct | i.e. adduct of p-(4,6-diamino-S-triazinyl-2-yl)aminophenyl arsenoxide with N1,N8-bis-(glutathionyl)spermidine | Leishmania donovani | |
| melarsen-trypanothione disulfide-adduct | - |
Leishmania donovani | |
| NaCl | below 5% remaining activity at 1 M | Leishmania donovani | |
| potassium antimony tartrate | assay containing 10% vol. methanol | Leishmania donovani | |
| triostam | assay containing 10% vol. methanol | Leishmania donovani | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
NADPH | - |
Leishmania donovani | |
| 0.036 | - |
trypanothione disulfide | - |
Leishmania donovani | |
| 0.115 | - |
glutathionylspermidine disulfide | - |
Leishmania donovani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50900 | - |
2 * 50900, SDS-PAGE | Leishmania donovani |
| 106000 | - |
gel filtration | Leishmania donovani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant overexpression of the enzyme from plasmid | Leishmania donovani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.2 | - |
crude extract of wild-type cells | Leishmania donovani |
| 2.1 | - |
crude extract cells overexpressing the recombinant enzyme | Leishmania donovani |
| 112.7 | - |
purified enzyme | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathionylspermidine disulfide + NADPH | - |
Leishmania donovani | 2-glutathionylspermidine + NADP+ | - |
? | |
| trypanothione disulfide + NADPH | trypanothione is the oxidized form of N1,N8-bis-(glutathionyl)spermidine, the latter form a stable adduct with melarsen oxide | Leishmania donovani | trypanothione + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50900, SDS-PAGE | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TR | - |
Leishmania donovani |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 179 | - |
trypanothione disulfide | - |
Leishmania donovani | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
additional 2 minor optima at pH 6.6 and pH 8.2 | Leishmania donovani |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | - |
Leishmania donovani |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Leishmania donovani | |
| NADPH | - |
Leishmania donovani | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
melarsen-trypanothione disulfide-adduct | - |
Leishmania donovani | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
