Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.8.1.11 extracted from

  • Yanagawa, H.
    Asparagusate reductase (1987), Methods Enzymol., 143, 516-521.
No PubMed abstract available
Search for more literature for 1.8.1.11

General Stability

General Stability Organism
EDTA ion stabilizes the enzyme Asparagus officinalis
PO43- stabilizes the enzyme Asparagus officinalis

Inhibitors

Inhibitors Comment Organism Structure
NADH above 0.1 mM Asparagus officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 potassium ferricyanide enzyme II Asparagus officinalis
0.9
-
 potassium ferricyanide enzyme I Asparagus officinalis
3
-
 lipoic acid
-
 Asparagus officinalis
20
-
 asparagusic acid
-
 Asparagus officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000 111000 enzyme I and II, sedimentation equilibrium Asparagus officinalis
112000
-
 enzyme I and II, gel filtration Asparagus officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
asparagusate + NADH Asparagus officinalis essential component of pyruvate dehydrogenase complex dihydroasparagusate + NAD+
-
 r

Organism

Organism UniProt Comment Textmining
Asparagus officinalis
-
 2 asparagusate dehydrogenases with lipoyl dehydrogenase activity
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, ion-exchange, ultracentrifugation, copurification of type I and II Asparagus officinalis

Reaction

Reaction Comment Organism Reaction ID
3-mercapto-2-mercaptomethylpropanoate + NAD+ = asparagusate + NADH + H+ exhibits lipoyl dehydrogenase activity Asparagus officinalis
a

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
 Asparagus officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.215
-
 enzyme/fraction II towards asparagusic acid Asparagus officinalis
0.425
-
 enzyme/fraction I towards asparagusic acid Asparagus officinalis

Storage Stability

Storage Stability Organism
-80°C, 67 mM sodium phosphate, pH 7.0, no loss of activity for at least 1 month Asparagus officinalis
4°C, 67 mM sodium phosphate, pH 7.0, very unstable Asparagus officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Asparagus officinalis 2 ferrocyanide + NAD+ + H+
-
 ?
asparagusate + NADH essential component of pyruvate dehydrogenase complex Asparagus officinalis dihydroasparagusate + NAD+
-
 r
asparagusic acid + NADH
-
 Asparagus officinalis dihydroasparagusate + NAD+
-
 r
lipoic acid + NADH
-
 Asparagus officinalis dihydrolipoic acid + NAD+
-
 r
NADH + H+ + potassium ferricyanide
-
 Asparagus officinalis NAD+ + potassium ferrocyanide
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Asparagus officinalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 5 min, activity completely retained Asparagus officinalis
50
-
 above, gradual loss of lipoyl dehydrogenase activity, complete loss at 90°C Asparagus officinalis
60 70 5 min, 1.4-fold activation of asparagusate dehydrogenase activity Asparagus officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.25
-
 for K3Fe(CN)6 reduction Asparagus officinalis
5.9
-
 asparagusic and lipoic acid reduction Asparagus officinalis

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein, approximately 1 mol of FAD per mol of protein Asparagus officinalis
NADH 0.1 mM optimal concentration for maximal activity Asparagus officinalis