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Literature summary for 1.7.7.2 extracted from

  • Srivastava, A.P.; Hardy, E.P.; Allen, J.P.; Vaccaro, B.J.; Johnson, M.K.; Knaff, D.B.
    Identification of the ferredoxin-binding site of a ferredoxin-dependent cyanobacterial nitrate reductase (2017), Biochemistry, 56, 5582-5592 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.7.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechococcus elongatus PCC 7942 = FACHB-805

Protein Variants

Protein Variants Comment Organism
E219Q the mutant shows 96.1% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
K201Q the mutant shows 14.8% activity and decreased binding affinity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
K614Q the mutant shows 11.5% activity and decreased binding affinity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
K614R the mutant shows 63.3% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
R197Q the mutant shows 91.8% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
R43K the mutant shows 91% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
R43Q the mutant shows 16.4% activity and decreased binding affinity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
R46K the mutant shows 97.7% activity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805
R46Q the mutant 24.3% activity and decreased binding affinity when reduced ferredoxin serves as the electron donor compared to the wild type enzyme Synechococcus elongatus PCC 7942 = FACHB-805

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
 nitrate mutant enzyme K614R, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0045
-
 nitrate mutant enzyme E219QR, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.006
-
 nitrate wild type enzyme, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0066
-
 nitrate mutant enzyme R46K, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.007
-
 nitrate mutant enzyme R197Q, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0081
-
 nitrate mutant enzyme R43K, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0096
-
 nitrate mutant enzyme K201R, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.038
-
 reduced ferredoxin wild type enzyme, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0419
-
 reduced ferredoxin mutant enzyme R197Q, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0422
-
 reduced ferredoxin mutant enzyme E219QR, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0445
-
 reduced ferredoxin mutant enzyme K614R, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0456
-
 reduced ferredoxin mutant enzyme K201R, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0486
-
 reduced ferredoxin mutant enzyme R43K, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.0496
-
 reduced ferredoxin mutant enzyme R46K, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.055
-
 nitrate mutant enzyme R46Q, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
0.364
-
 reduced ferredoxin mutant enzyme R46Q, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme contains 3.6 moles iron per mole of enzyme Synechococcus elongatus PCC 7942 = FACHB-805
Molybdenum the enzyme contains 0.93 moles molybdenum per mole of enzyme Synechococcus elongatus PCC 7942 = FACHB-805

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrate + 2 reduced ferredoxin + 2 H+ Synechococcus elongatus PCC 7942 = FACHB-805
-
 nitrite + H2O + 2 oxidized ferredoxin
-
 ?

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5
-
 mutant enzyme K614Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
4.5
-
 mutant enzyme K201Q, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
5
-
 mutant enzyme R43Q, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
7.4
-
 mutant enzyme R46Q, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
19.3
-
 mutant enzyme K614R, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
27.8
-
 mutant enzyme R43K, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
27.9
-
 mutant enzyme R197Q, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
29.3
-
 mutant enzyme E219Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
29.4
-
 mutant enzyme K201R, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
29.8
-
 mutant enzyme R46K, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
30.5
-
 wild type enzyme, with reduced ferredoxin as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
721
-
 mutant enzyme R43Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
734
-
 mutant enzyme R43K, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
738
-
 mutant enzyme R197Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
749
-
 mutant enzyme E219Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
761
-
 mutant enzyme K614R, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
768
-
 mutant enzyme R46Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
771
-
 mutant enzyme K614Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
775
-
 mutant enzyme K201R, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
779
-
 mutant enzyme K201Q, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
783
-
 mutant enzyme R46K, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805
790
-
 wild type enzyme, with reduced methyl viologen as electron donor, pH and temperature not specified in the publication Synechococcus elongatus PCC 7942 = FACHB-805

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrate + 2 reduced ferredoxin + 2 H+
-
 Synechococcus elongatus PCC 7942 = FACHB-805 nitrite + H2O + 2 oxidized ferredoxin
-
 ?
nitrate + 2 reduced methyl viologen + 2 H+
-
 Synechococcus elongatus PCC 7942 = FACHB-805 nitrite + H2O + 2 oxidized methyl viologen
-
 ?

Subunits

Subunits Comment Organism
? x * 78000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805

Synonyms

Synonyms Comment Organism
Fd-dependent nitrate reductase
-
 Synechococcus elongatus PCC 7942 = FACHB-805
narB
-
 Synechococcus elongatus PCC 7942 = FACHB-805