Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 1 mM, 68% loss of activity | Haloferax mediterranei | |
cyanide | 1 mM, 76% loss of activity | Haloferax mediterranei | |
Dithionite | 1 mM, 24% loss of activity | Haloferax mediterranei | |
EDTA | 1 mM, 22% loss of activity | Haloferax mediterranei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
methyl viologen | - |
Haloferax mediterranei | |
0.82 | - |
nitrate | - |
Haloferax mediterranei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Haloferax mediterranei | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains Fe-S clusters. NarB codes for a 219-amino-acid-residue iron Rieske protein. | Haloferax mediterranei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Haloferax mediterranei | the regulation of the nar gene occurs at transcriptional level induced by oxygen-limiting conditions and the presence of nitrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax mediterranei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloferax mediterranei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Haloferax mediterranei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the regulation of the nar gene occurs at transcriptional level induced by oxygen-limiting conditions and the presence of nitrate | Haloferax mediterranei | ? | - |
? | |
nitrate + reduced methyl viologen | reduced methyl viologen is the best electron donor. NADPH or NADH show only 2.4% of maximal activity, no activity with FADH2 | Haloferax mediterranei | nitrite + methyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
respiratory nitrate reductase | - |
Haloferax mediterranei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
at 3.4 M NaCl | Haloferax mediterranei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
at 40°C | Haloferax mediterranei |
8.2 | - |
at 60°C | Haloferax mediterranei |