Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Synechocystis sp. |
Crystallization (Comment) | Organism |
---|---|
NMR-study of protein-protein interaction of ferredoxin and nitrite reductase shows three acidic regions of ferredoxin to be major sites for the interaction with the enzyme, indicating that the complex is stabilized through electrostatic interaction | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0077 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant E94Q/E95Q, pH 7.5, 25°C | Synechocystis sp. | |
0.0097 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin, pH 7.5, 25°C | Synechocystis sp. | |
0.0213 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant D67N/D68N/D69N, pH 7.5, 25°C | Synechocystis sp. | |
0.0478 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant E31Q/E32Q/D36N, pH 7.5, 25°C | Synechocystis sp. | |
0.0746 | - |
reduced ferredoxin | substrate maize ferredoxin, pH 7.5, 25°C | Synechocystis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + 6 reduced ferredoxin | - |
Synechocystis sp. | ammonia + 2 H2O + 6 oxidized ferredoxin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
101.4 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant E94Q/E95Q, pH 7.5, 25°C | Synechocystis sp. | |
138.2 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin, pH 7.5, 25°C | Synechocystis sp. | |
178.3 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant D67N/D68N/D69N, pH 7.5, 25°C | Synechocystis sp. | |
203.2 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant E31Q/E32Q/D36N, pH 7.5, 25°C | Synechocystis sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4300 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant E31Q/E32Q/D36N, pH 7.5, 25°C | Synechocystis sp. | |
8400 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant D67N/D68N/D69N, pH 7.5, 25°C | Synechocystis sp. | |
13200 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin mutant E94Q/E95Q, pH 7.5, 25°C | Synechocystis sp. | |
14200 | - |
reduced ferredoxin | substrate Leptolyngbya boryana ferredoxin, pH 7.5, 25°C | Synechocystis sp. |