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Literature summary for 1.7.7.1 extracted from

  • Bellissimo, D.B.; Privalle, L.S.
    Expression of spinach nitrite reductase in Escherichia coli: site-directed mutagenesis of predicted active site amino acids (1995), Arch. Biochem. Biophys., 323, 155-163.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
fusion with beta-galactosidase Spinacia oleracea

Protein Variants

Protein Variants Comment Organism
C514S almost complete loss of activity Spinacia oleracea
C518S almost complete loss of activity Spinacia oleracea
G513A increased Km for nitrite compared to the wild type enzyme Spinacia oleracea
G513E loss of most of the activity Spinacia oleracea
G513V loss of most of the activity Spinacia oleracea
G519A 68% activity of wild type enzyme Spinacia oleracea
G519E 8% activity of wild type enzyme Spinacia oleracea
G519V 9% activity of wild type enzyme Spinacia oleracea
P515A marginal loss of activity Spinacia oleracea
P515S marginal loss of activity Spinacia oleracea
P515T marginal loss of activity Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
nitrite
-
Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
SDS-PAGE Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
-
Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + reduced ferredoxin
-
Spinacia oleracea ammonia + oxidized ferredoxin
-
?

Cofactor

Cofactor Comment Organism Structure
siroheme
-
Spinacia oleracea