Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | enzyme from higher plants and algae | [Chlorella] fusca | |
CO | enzyme from higher plants and algae | Cucurbita pepo | |
CO | enzyme from higher plants and algae | Neopyropia yezoensis | |
CO | enzyme from higher plants and algae | Spinacia oleracea | |
cyanide | - |
[Chlorella] fusca | |
cyanide | - |
Cucurbita pepo | |
cyanide | - |
Neopyropia yezoensis | |
cyanide | - |
Spinacia oleracea | |
Mersalyl | - |
Cucurbita pepo | |
p-hydroxymercuribenzoate | enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not | [Chlorella] fusca | |
p-hydroxymercuribenzoate | enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not | Cucurbita pepo | |
p-hydroxymercuribenzoate | enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not | Neopyropia yezoensis | |
p-hydroxymercuribenzoate | enzyme from higher plants and eukaryotic algae inhibited, cyanobacterial enzyme not | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Spinacia oleracea | |
additional information | - |
additional information | - |
[Chlorella] fusca | |
additional information | - |
additional information | - |
Cucurbita pepo | |
additional information | - |
additional information | - |
Neopyropia yezoensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
particle-bound | chlorophyll-containing subcellular particle | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
soluble | exception: Anacystis | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
[Chlorella] fusca | |
Iron | - |
Cucurbita pepo | |
Iron | - |
Neopyropia yezoensis | |
Iron | enzyme contains 1 tetranuclear center (4Fe-4S) and 1 siroheme, minimum of 5 Fe atoms per active enzyme molecule | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | 63000 | gel filtration, sedimentation equilibrium | Spinacia oleracea |
63000 | - |
- |
Spinacia oleracea |
63000 | - |
- |
[Chlorella] fusca |
63000 | - |
- |
Cucurbita pepo |
63000 | - |
- |
Neopyropia yezoensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced ferredoxin | Synechococcus elongatus PCC 7942 = FACHB-805 | - |
ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | Spinacia oleracea | - |
ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | [Chlorella] fusca | - |
ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | Cucurbita pepo | - |
ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | Neopyropia yezoensis | - |
ammonia + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cucurbita pepo | - |
- |
- |
Neopyropia yezoensis | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Synechococcus elongatus PCC 7942 = FACHB-805 | - |
- |
- |
[Chlorella] fusca | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Synechococcus elongatus PCC 7942 = FACHB-805 | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Spinacia oleracea | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | [Chlorella] fusca | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Cucurbita pepo | |
NH3 + 2 H2O + 3 oxidized ferredoxin = nitrite + 3 reduced ferredoxin + 7 H+ | mechanism | Neopyropia yezoensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + reduced ferredoxin | - |
Synechococcus elongatus PCC 7942 = FACHB-805 | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
[Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | - |
Neopyropia yezoensis | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | other electron donor: methyl viologen | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | other electron donor: methyl viologen | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | other electron donor: methyl viologen | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | other electron donor: methyl viologen | Neopyropia yezoensis | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | other electron donors: flavodoxin | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | other electron donors: flavodoxin | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | other electron donors: flavodoxin | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | other electron donors: flavodoxin | Neopyropia yezoensis | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | Spinacia oleracea | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | [Chlorella] fusca | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | Cucurbita pepo | ammonia + oxidized ferredoxin | - |
? | |
nitrite + reduced ferredoxin | enzyme can also catalyze the reduction of hydroxylamine to ammonia, at lower rate | Neopyropia yezoensis | ammonia + oxidized ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Spinacia oleracea |
monomer | - |
[Chlorella] fusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
nitrite | - |
Neopyropia yezoensis | |
54 | - |
nitrite | - |
[Chlorella] fusca | |
89 | - |
nitrite | - |
Cucurbita pepo | |
110 | - |
nitrite | - |
Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Spinacia oleracea |
7.5 | - |
- |
[Chlorella] fusca |
7.5 | - |
- |
Cucurbita pepo |
7.5 | - |
- |
Neopyropia yezoensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
siroheme | prosthetic group | Spinacia oleracea | |
siroheme | 1 mol siroheme per mol of enzyme | Spinacia oleracea |