General Stability | Organism |
---|---|
freezing in liquid nitrogen may be repeated up to six times, with a reduction of 20% of the activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | 5 mM, 95-98% inhibition | Escherichia coli | |
cyanide | 1 mM, 95-98% inhibition | Escherichia coli | |
additional information | p-chloromercuribenzoate (0.5 mM) or 2-heptyl-4-hydroxyquinolin N-oxide (1 mM) are almost without effect on the purified enzyme tested with reduced viologen as electron donor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
nitrate | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | the 230000 Da complex contains 13 atoms iron and 12 atoms labile sulfur/molecules | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 150000 (alphaz) + 1 * 60000 (betaz) + a b-type cytochrome subunit, SDS-PAGE | Escherichia coli |
150000 | - |
1 * 150000 (alphaz) + 1 * 60000 (betaz) + a b-type cytochrome subunit, SDS-PAGE | Escherichia coli |
230000 | - |
alpha(Z)beta(Z) complex, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | nitrate reductase Z expression is regulated in a manner opposite to that of nitrate reductase A. The narGHJZ operon is aerobically repressed, strongly induced by nitrate and positively regulated by the fnr gene product. The expression of narZ is anaerobically repressed, induced weakly, if at all, by nitrate and negatively regulated by the fnr gene product. The opposing regulation of these two enzymes suggests that a function of nitrate reductase Z may be to catalyse the immediate flow of electrons to nitrate during an aerobic/anaerobic transition when the bacterium is grown in the presence of nitrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is remarkably resistant to air inactivation since only 2-5% of the activity is lost after a 1 h treatment | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, slow freezing leads to a 30% loss of activity | Escherichia coli |
4°C, the purified preparation can be stored up to three days without inactivation | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | nitrate reductase Z expression is regulated in a manner opposite to that of nitrate reductase A. The narGHJZ operon is aerobically repressed, strongly induced by nitrate and positively regulated by the fnr gene product. The expression of narZ is anaerobically repressed, induced weakly, if at all, by nitrate and negatively regulated by the fnr gene product. The opposing regulation of these two enzymes suggests that a function of nitrate reductase Z may be to catalyse the immediate flow of electrons to nitrate during an aerobic/anaerobic transition when the bacterium is grown in the presence of nitrate | Escherichia coli | ? | - |
? | |
additional information | bromate and chlorate are substrates of the enzyme | Escherichia coli | ? | - |
? | |
nitrate + reduced benzyl viologen | - |
Escherichia coli | nitrite + oxidized benzyl viologen + H2O | - |
? | |
nitrate + reduced methyl viologen | - |
Escherichia coli | nitrite + oxidized methyl viologen + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 1 * 150000 (alphaz) + 1 * 60000 (betaz) + a b-type cytochrome subunit, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
nitrate reductase Z | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
bis(molybdopterin guanine dinucleotide)molybdenum cofactor | molybdoenzyme | Escherichia coli | |
cytochrome | although the spectral studies of nitrate reductase Z reveals the presence of a b-type cytochrome subunit (1.5 mol/molecule of 230000 Da), none can be detected in the SDS-PAGE | Escherichia coli |