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Literature summary for 1.7.3.3 extracted from

  • Fang, Z.; Liu, Y.; Liu, J.; Sun, R.; Chen, H.; Gao, X.; Yao, W.
    Designing and engineering of a site-specific incorporation of a keto group in uricase (2011), Chem. Biol. Drug Des., 78, 353-360.
    View publication on PubMed

Application

Application Comment Organism
pharmacology urate oxidase is a potential therapeutic protein in the prevention and treatment of tumor lysis syndrome and hyperuricemia. However, its severe immunogenicity limits its clinical application. Engineering site-specific modifications of keto groups in urate oxidase by using evolved Methanocaldococcus jannaschii aminoacyl-tRNA synthetase(s)/suppressor tRNA pairs reduces its antigenicity. The mutated uricase exhibits decreased antigenic properties, while its catalytic activities remain unchanged Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
Urate oxidase
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Methanocaldococcus jannaschii