Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.3.3 extracted from

  • Collings, I.; Watier, Y.; Giffard, M.; Dagogo, S.; Kahn, R.; Bonnete, F.; Wright, J.P.; Fitch, A.N.; Margiolaki, I.
    Polymorphism of microcrystalline urate oxidase from Aspergillus flavus (2010), Acta Crystallogr. Sect. D, 66, 539-548.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae Aspergillus flavus

Crystallization (Commentary)

Crystallization (Comment) Organism
ligand-free Uox crystallized with NH4Cl and 15% (w/v) PEG 8000, ligand-free Uox crystallized in water with 10% (w/v) PEG 8000, ligand-free Uox crystallized with NaCl and 15% (w/v) PEG 8000, ligand-free Uox crystallized with (NH4)2SO4 and 15% (w/v) PEG 8000, ligand-free Uox crystallized with NaCl and 8% PEG 8000, ligand-free Uox crystallized with KCl and 10% (w/v) PEG 8000, and Uox complexed with 8-azaxanthine and crystallized with NaCl and 10% (w/v) PEG 8000, in 50 mM Tris buffer pH 8.0 Aspergillus flavus

Inhibitors

Inhibitors Comment Organism Structure
8-Azaxanthine
-
Aspergillus flavus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
-
Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus Q00511
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex S200PG gel filtration Aspergillus flavus

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Aspergillus flavus

Synonyms

Synonyms Comment Organism
Rasburicase
-
Aspergillus flavus
Uox
-
Aspergillus flavus
Urate oxidase
-
Aspergillus flavus