Application | Comment | Organism |
---|---|---|
medicine | urate oxidase is used to reduce toxic urate accumulation during chemotherapy | Aspergillus flavus |
Crystallization (Comment) | Organism |
---|---|
crystals of about a few tens of micrometres in size, which is nucleated previously in crystallization batch containing 5% PEG 8000, 100 mM NaCl, 8 mg/ml uox-substrate complex and 100 mM Tris-HCl pH 8.5, are used as seeds and their size and quality are further improved using a temperature-control device, large crystals of Uox, co-crystallized with its substrates analogues 8-azaxanthine, 9-methyluric acid or the natural substrate in the presence of cyanide (0.5-2 mg/ml), and soaks with the natural substrate in the absence of cyanide, diffracting to high resolutions are obtained, in the presence of different inhibitors, the crystal form of Uox has a body-centred orthorhombic symmetry and one of the largest primitive unit-cell volumes (a: 80 A, b: 96 A, c: 106 A) | Aspergillus flavus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-Azaxanthine | substrate analogue binds in the active site of the enzyme | Aspergillus flavus | |
9-methyluric acid | substrate analogue | Aspergillus flavus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
137000 | - |
for the homotetramer, determined by neutron crystallographic analysis | Aspergillus flavus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | Aspergillus flavus | purine metabolism | 5-hydroxyisourate + H2O2 | a metastable intermediate, which is further degrades to allantoin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Aspergillus flavus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | purine metabolism | Aspergillus flavus | 5-hydroxyisourate + H2O2 | a metastable intermediate, which is further degrades to allantoin | ? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | determined by X-ray crystallography, formed by crystallographic symmetry operations | Aspergillus flavus |
Synonyms | Comment | Organism |
---|---|---|
Uox | - |
Aspergillus flavus |
Urate oxidase | - |
Aspergillus flavus |