Cloned (Comment) | Organism |
---|---|
a plasmid containing the AgUOX gene is introduced into the expression host cell Escherichia coli DH1 | Arthrobacter globiformis |
Crystallization (Comment) | Organism |
---|---|
crystallizations are performed using the hanging-drop vapour-diffusion method at 19.9°C, structures of crystals soaked with the substrate uric acid, the inhibitor 8-azaxanthin and allantoin are determined at 1.9-2.2 A resolution, 2 homotetramers comprise the asymmetric crystallographic unit, each subunit contains 2 T-fold domains of topology, which are usually found in purine- and pterin-binding enzymes, the uric acid substrate is bound tightly to the enzyme by interactions with Arg180, Leu222 and Gln223 from one subunit and with Thr67 and sp68 of the neighbouring subunit in the tetramer | Arthrobacter globiformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-Azaxanthine | substrate analogue | Arthrobacter globiformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | Arthrobacter globiformis | ureide pathway | 5-hydroxyisourate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | D0VWQ1 | - |
- |
Purification (Comment) | Organism |
---|---|
by anion exchange and hydropbobic interaction chromatography | Arthrobacter globiformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | ureide pathway | Arthrobacter globiformis | 5-hydroxyisourate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | determined by X-ray diffraction measurements, the AgUOX-native structure is solved by molecular replacement using the program AmoRe, pairs of dimers are stacked face-to-face to form a tetramer | Arthrobacter globiformis |
Synonyms | Comment | Organism |
---|---|---|
AgUOX | - |
Arthrobacter globiformis |
Urate oxidase | - |
Arthrobacter globiformis |
uricase | - |
Arthrobacter globiformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | UOX is unique among the oxygen-requiring enzymes in the sense that it does not need a cofactor to convert uric acid to 5-hydroxyisourate | Arthrobacter globiformis |