Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.3.3 extracted from

  • Retailleau, P.; Colloc'h, N.; Vivares, D.; Bonnete, F.; Castro, B.; El Hajji, M.; Prange, T.
    Urate oxidase from Aspergillus flavus: new crystal-packing contacts in relation to the content of the active site (2005), Acta Crystallogr. Sect. D, 61, 218-229.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Aspergillus flavus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-difusion method. Four different crystal forms of Uox are analyzed. In the presence of uracil and 5,6-diaminouracil crystals usually belong to the trigonal space group P3(1)21, the asymmetric unit of which contains one tetramer of Uox. Chemical oxidation of 5,6-diaminouracil within the protein may occur, leading to the canonical (I222) packing with one subunit per asymmetric unit. Coexistence of two crystal forms, P2(1) with two tetramers per asymmetric unit and I222, is found in the same crystallization drop containing another inhibitor, guanine. A fourth form, P2(1)2(1)2 with one tetramer per asymmetric unit, results in the presence of cymelarsan, an additive Aspergillus flavus

Organism

Organism UniProt Comment Textmining
Aspergillus flavus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aspergillus flavus