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Literature summary for 1.7.2.6 extracted from
- Heterotrophic nitrogen removal in Bacillus sp. K5 involvement of a novel hydroxylamine oxidase (2017), Water Sci. Technol., 76, 3461-3467 .
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. K5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sepharose CL-6B column chromatography and Sephacryl S-100 gel filtration | Bacillus sp. K5 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.003 | - |
at pH 9.0 and 30°C | Bacillus sp. K5 |
| 0.006 | - |
at pH 8.0 and 40°C | Bacillus sp. K5 |
| 0.008 | - |
at pH 7.0 and 30°C | Bacillus sp. K5 |
| 0.014 | - |
at pH 8.0 and 25°C | Bacillus sp. K5 |
| 0.015 | - |
at pH 7.5 and 30°C | Bacillus sp. K5 |
| 0.015 | - |
at pH 8.0 and 37°C | Bacillus sp. K5 |
| 0.022 | - |
at pH 8.0 and 30°C | Bacillus sp. K5 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxylamine + H2O + ferricyanide | - |
Bacillus sp. K5 | nitrite + ferrocyanide + H+ | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 71000, SDS-PAGE | Bacillus sp. K5 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAO | - |
Bacillus sp. K5 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bacillus sp. K5 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | 57.32% activity at 25°C, 100% activity at 30°C, 61.64% activity at 37°C, 26.52% activity at 40°C. There is no activity at temperatures above 40°C | Bacillus sp. K5 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus sp. K5 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | 35.4% activity at pH 7.0, 100% activity at pH 8.0, 13.86% activity at pH 9.0. No activity is detected below pH 7.0 or above pH 9.0 | Bacillus sp. K5 |
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Release 2023.1 (January 2023)
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